Media
LIM broth is an example of _______ medium
Medium that is 0.3-0.4% agar
What is semisolid?
Methyl Red requires what incubation
Phenol Red Carbohydrate broth is incubated in
1) -25 2) 35 3) ambient 4) CO2
What is 35 and ambient?
A yellow ONPG test indicates production of
beta-galactosidase
This type of media would be used to cultivate N. gonorrhoeae from normal flora
The cytochrome oxidase test is used primarily for the identification of
gram-negative organisms
Acetyl-Methyl Carbinol (acetoin) is the basis of _______ test
Stable acid is the basis of ________ test
What is Voges-Proskauer?
What is Methyl Red?
An organism that requires carbon dioxide/ does not grow in ambient or anaerobic environments
What is capnophilic?
Colistin-nalidixic agar (CNA) is used to recover what types of organisms & give an example
What is Gram positive, S. aureus?
An oxidase-negative, gram-negative rod turns the urea agar slant pink within 4 hours. This indicates what species
Proteus
Klebsiella oxytoca can be distinguished from Klebsiella pneumoniae by this biochemical reaction
What is Indole
Microaerophilic is broken down into
___% oxygen
___% carbon dioxide
__% nitrogen
5% Oxygen
10% Carbon Dioxide
85% Nitrogen
Which reactions is associated with the gram-negative rod best known for its capsule production?
VP positive
Phenylalanine deaminase is produced by all of the following organisms except
Serratia marcescens.
Which of the following organisms always requires antibiotic therapy when isolated in a stool culture?
Salmonella typhi
About nitrate reduction: The tube is colorless.
To test for nitrate reduction to nitrogen gas, you add
What is zinc dust?
Which of the following antigens remains after boiling a suspension of a member of the Enterobacteriaceae for 10 minutes?
O antigen
Which of the following warrant a thioglycolate broth?
Shunt
Middle ear aspirate
Cervical swab
Rectal Swab
What is shunt?
Motility at 25°C: positive PAD: no color change
Motility at 35°C: positive Indole: no color change
Urea: no color change VP: no color change
If this organism is a strict pathogen, you expect it to also be
citrate _______ and lysine ______
citrate positive and lysine positive.
What are the active ingredients in CIN agar?
Cefsulodin-Irgasan-Novobiocin (CIN)
You are testing for acetoin:
(1) ____ mL of _____ alpha naphthol
(2)____mL of _____ KOH
(1) 0.6 mL of 5.0% alpha naphthol
(2) 0.2 mL of 40% KOH
What Enterobacteriaceae can be enriched by incubation at 4C?
Yersinia Enterocolitica
This is the media that an isolate should be cultured in prior to indole testing
What is Tryptophan broth?