Weigh-Out
Finished product Twix, Combos, and M&M's all have one thing in common.
What is they are processed in STR?
Fraser Broth refers to the secondary enrichment for which machine method.
What is Listeria Vidas?
This statement will appear on the final COA if the client request any changes to testing that stray from the standard SOP.
What is a deviation disclaimer?
What is steam?
A specific type of PPE that must be worn whenever handling sample or sample materials including media.
What is a lab coat?
This is the amount of time you have between hydrating samples and pipetting them.
What is 15 minutes?
This is the name of the maintenance kit that must be preformed on the vidas machines bimonthly to detect abnormalities in the instruments pipette mechanisms and optical systems.
What is QCV?
No matter the scenario. This team of knowledgeable Deibel associates have to be contacted before the client in the event of presumptive client results.
Who is the Presumptives team?
This part of the lot number aides in the traceability of the media. It would allow an auditor to easily understand which run of the day the lot was.
What is the batch number?
This is something that is done at the beginning and end of the day in each area around the lab.
What is temps?
Vidas, PCR, and Gene-up. These are all ----------- test.
What is qualitative .
The GENE-UP platform requires this this daily before samples are run.
What is the Self Test?
What must be done at the end of each month to prepare cultures for use next month?
What is purity checks?
What is the standard autoclave cycle for a 3 liter jug of media?
What is 45 min at 121 degrees Celsius?
This SOP outlines the process of shipping samples, cultures, and equipment including documentation requirements, packing needs, and preferred vendors.
What the 159 or Chain of Custody?
You receive a client SARF indicating the sample requires salmonella real time pcr testing. This is a test that you are largely unfamiliar with and wonder is it the same as standard Bax pcr testing. Where do you go to find information about processing this sample?
What is the SOP or Sharepoint to look up SOP?
This type of method control error on PCR commonly occurs if there are machine problems, missing pellets, or if the machine needs to be cleaned. The graphs usually has no peaks or internal control lines.
What is signal error?
This set of plates point to a ________ salmonella presumptive.
XLD: yellow colonies with no black centers
HEK: orange/pink colonies with no black centers.
BS: Dull brown or green colonies with no metallic sheen.
Chrome: Blue colonies.
What is atypical salmonella presumptive?
Extra 100 points to whoever can tell if its atypical H2S positive, atypical salmonella, or atypical non-salmonella growth.
------ must be run weekly in every autoclave including the market forge.
What is spores?
This document holds all information regarding the Deibel life cycle of any supply item that we receive. This includes vendor info, open date, storage area.
What is the 1111-1.
When receiving samples, what is the expected order that samples should be checked-in based on risk levels?
What important step happens on day 1 of Listeria USDA testing that does not occur on day 1 of Listeria FDA testing?
What is transferring to MOPS-BLEB in addition to streaking to MOX?
Environmental Listeria pcr presumptive with the following reactions:
Typical growth on MOX/OX/ALOA. No halos on ALOA. Isolates taken to Motility and TSB-YE. All isolates were catalase positive and showed tumbling but Umbrellas are still growing and not ready to call.
Give the next step.
What is set up an API?
This salmonella secondary enrichment media is autoclaved at a lower temperature to help preserve the selectivity of the medium while still allowing for the survival of the target organism. What is the media and what is the temperature?
What is RV and 115 degree C?
What priority level would you assign the following incident? Who should be alerted?
You arrive to the lab one morning and see you are scheduled to work in transfers. You arrive in your area and note that the PCR machines are turned off which strikes you as odd but you don't think twice and turn them back on. Once on you realize that the sample runs from the previous night never completed and that the machine alerts you that it lost power at some point in the last night. You alert your shift lead thinking the building just lost power overnight and they prompt you to rerun the samples. You do as told and set samples up to rerun. Later that day you approach the PCR machine because you think the run time should have passed. You notice again that the run did not complete and the machine states that it has lost power only this time you are concerned because you have been in the lab all day and the power never went out. You again notify your shift lead and the two of you realize you will not be able to get the client results in time as the machines are not working.
What is a priority level 2 (or priority level 1 b/c when in doubt escalate to next level)? Who is site level management and director level associate via phone call or text?
This issue poses serious risk to Deibel and the client if not properly addressed. We have alternative methods we could run samples on with client permission but we would want to notify a director to help us figure out how best to notify client since there will be a delay in results and a new method listed on COA. Also, some clients may need results in a certain method and a director can help coordinate with another lab if samples need to be redirected until PCR machines were fixed which a director could help coordinate.