Waste Prep
Why do we limit bags to 20 lbs?
Ensures steam penetration, prevents unsterilized waste (BG's validation)
How often must a chemical (sterigage) indicator be run?
Once per day - confirms sterilization conditions
How many bins should be loaded onto the autoclave rack?
8 bins - ensures proper spacing for steam circulation and efficient use of runs
What must the pressure gauge read before opening the autoclave?
0 PSI - prevents explosive release of steam and severe burns
Why do we have a designated dumpster for autoclave trash?
Designated dumpster - having a full dumpster will cause "over-the-head" lifting that is very unsafe if the liquid is still hot (and ew gross even if its not hot)
What’s the risk of tightly packed or sealed bags?
Blocks steam and waste may not be sterile
What must be on for the autoclave to run properly?
Jacket - circulates steam between the outer shell and the inner vessel. Maximizes efficiency, temperature uniformity, and reduces condensation
The autoclave is turned off at the end of every day (ideally). Why? 2 reasons
To preserve the life of the autoclave and to ensure it is cool when checking the filter the next day
Name the required pieces of PPE when opening the autoclave and disposing of trash. (4 things)
Face shield / heat/water-resistant gloves / lab coat / apron - protects from steam and hot liquid exposure
When are aprons, gloves and face shields disinfected?
After each use
Why keep bleach away from autoclave loads?
Can release toxic fumes when heated
What should you do if the chemical indicator or printer paper fails?
Notify supervisor/management
I forgot to check the chamber drain filter first thing and now it is not cool, what should I do?
Turn the jacket off and allow to cool before checking (appx 15-20 minutes)
Why should you not stand directly in front of the autoclave when opening it?
Avoids direct exposure to escaping steam
Why should autoclaved waste not be allowed to accumulate?
Reduces contamination risk and keeps workflow safe
Why should bins not be overstacked or uneven?
Prevents tipping and biohazard spills during transport and loading
What is the pressure range for autoclave trash?
17-25 PSI - allows proper steam temperature by elevating boiling water in a way that doesn't evaporate, properly killing microorganisms
What should you do if proper cart alignment cannot be achieved, the rack becomes unstable, or the wheels are misaligned?
Stop and notify a supervisor - prevents tipping, spills, and injury
Why should we allow the autoclaved trash to sit for at least 15 minutes?
The liquid has been proven to be up to 160F immediately after removing from the autoclave, this can cause severe burns and scalding within seconds of direct contact
How often should the autoclave room be cleaned with bleach?
Daily (when used) - minimizes environmental contamination
Spray counter with 70% Alcohol, let air dry
What is the correct prompt cleaning sequence for sample buckets?
10% bleach (10 min) → rinse → 70% alcohol → air dry
ensures proper disinfection and prevents contamination spread
ensure that bleach is properly mopped up - do not create a slip hazard
What does a passing chemical indicator confirm?
That proper sterilization conditions were reached — reduces risk of biohazard exposure
What cycle and minimum temp and pressure conditions are required for sterilization?
Gravity cycle, 250°F, 17 PSI
When can standard nitrile gloves be used instead of heat/water resistant gloves?
When materials have cooled to a safe temperature (<120°F, ~1 hour)
When is biological ampule testing required? (2 things)
Monthly and after maintenance - confirms actual microbial kill, not just conditions