FAQ
How long do the instruments take to count?
In less than 30 seconds, the Countess 3 will:
autofocus, autolighting, image capture, and counting an area of almost four squares, increasing accuracy by reducing the variation of cell distribution.
True or False: The Countess 3 incorporates artificial intelligence.
True: The algorithm was taught to recognize cells by expert cell biologists based on hundreds of plates containing dozens of different cell types and sample conditions.
Name 3 applications beyond cell counting:
1. Detect different stages of apoptosis cell death 2. Measure transfection efficiency 3. Single-cell data 4. Counting isolated nuclei
What is the standard warranty that comes with Countess 3/3 FL Automated Cell Counters?
Standard 1-year warranty. During the 1-year system warranty period, all costs for shipping, labor, and parts for repairs are covered.
Can I buy the Countess 3 FL Instrument without Light Cubes?
Yes, however, light cubes can easily be added to the instrument as needed.
What are the advantages of using an automated cell counter over traditional manual counting methods?
How many optical detection channels does the Countess 3 FL have?
1 brightfield + 2 fluorescence = 3 total optical detection channels
Which service should you recommend for labs that operate under critical timelines?
Rapid Exchange: Replacement shipped out in 1 business day. 2 years (total 3 years)
Do the Countess 3 and Countess 3 FL provide count results that account for the 1:1 dilution of trypan blue to samples?
Yes, the 1:1 dilution is automatically accounted for by the Countess 3 and Countess 3 FL.
How do advanced algorithms in the Countess 3 enhance the accuracy of cell counts?
Automatically exclude debris and accurately identify cell boundaries within clumps, distinguishing between singlets and aggregates to provide reliable cell counts.
Why is counting isolated nuclei important?
Nuclei isolation allows characterization of heterogeneity among samples for single-cell assays like gene expression or ATAC sequencing.
When would a customer need AB Repair Center (ABRC) Support?
Regulated labs that must maintain asset tagging consistency to comply with regulated protocols
What range of cell sizes do the Countess 3 and Countess 3 FL Automated cell counters count?
Accurate counts for cells between 4 - 60 µm can be obtained
What role does the deep-learning neural network algorithm play in the Countess 3 Automated Cell Counter's ability to count cells accurately and precisely?
The algorithm can distinguish cells from debris and handle complex sample conditions.
True or False: Only the GFP, RFP, and Texas Red EVOS light cubes work with the Countess 3
False: With 14 interchangeable light cubes to choose from, the Countess 3 FL instrument is not limited to detection of cells expressing GFP and RFP.
What count-to-count precision should be expected with the Countess 3 and Countess 3 FL Automated Cell Counters?
The count-to-count specification is <10%. However, CV values of <%5 are routine due to the advanced focus and analysis algorithms.
Will AI continue to learn with new cell samples?
No, the algorithms are static. The algorithms were taught to optimize focus, lighting, and distinguish individual cells as live, dead, and in clumps.
What are Invitrogen ReadyCount reagents?
Bench-stable fluorescent stains: ReadyCount Red Dead Cell Stain (for staining dead nucleated cells), ReadyCount Blue Nuclear Stain, ReadyCount Green/Red Dead Viability Stain