Bacteria I
Bacteria II
Bacteria III
Bacteria IV
Lab Diagnosis
100

Specimen required to diagnose Whooping Cough and which bacteria is the culprit?

What is nasopharyngeal swab and Bordetella pertussis?

100

Bacteria causing infection after milking cows?

What is Brucella abortus?

100

Toxin testing is the most definitive test for this disease state.

What is Diptheria?

100

Gram stain from a thigh wound showing Gram-positive bacilli with spores.  The isolate did not grow on Brain Heart Infusion agar incubated aerobically.

What is Clostridium perfringens?

100

Urea Breath Test

What is the test for Helicobacter pylori?

200

42 degrees C

What is the optimal incubation temperature for Campylobacter?

200

Specimen used to isolate diptheroids

What is skin?

200

A method that should NOT be used when trying to cultivate an anaerobe

What is using metal loops, using aerobic conditions and media, incubating for only 24 hr

200

An acceptable anaerobic specimen from an abscess

What is an aspirate?

200

Mercury droplets

What B. pertussis looks like on Bordet Gengou

300

Isolated from a cutaneous black lesion.  No growth on MAC, 3+ growth on BA.  Colonies were non-hemolytic, nonmotile and large with off-white ground glass appearance with an irregular edge.  Gram stain showed a large, Gram positive rectangular bacilli

What is Bacillus anthracis?

300

Antitoxin

What is treatment for botulism?

300

College student at a beach party consumed raw oysters and other shellfish.  Presented at the eR the next day with symptoms of septicemia.  

oxidase positive, MAC - pink colonies gram negative bacilli

Vibrio vulnificus


300

Added to the medium to grow and differentiate Bacteroides from other anaerobes

What is 20% bile?

300

TCBS

Vibrio 

400

Causative agent of rice water stools

What is Vibrio cholera?

400

A gram negative coccobacillus which causes a chancroid and is sexually transmitted.

What is Haemophilus ducreyi?

400

Francisella tularensis, Pastuerella multocida, Brucella are all causative agents of what type of infections?

What is zoonotic?

400

Diagnostic of Clostridium botulinum

What is detection of toxin in the serum?

400

Blood Glucose Cystine Agar

What is media used to isolate Francisella?

500

Patient presents with leg wound infection after swimming in the ocean.  Direct smear from wound shows curved Gram negative bacilli.  Culture grows clear colonies on TCBS.  What is the diagnosis?

Vibrio vulnificus

500

Isolated from a bite wound from a pet cat, this bacteria has bipolar staining, oxidase positive, catalase positive, motility negative and no growth on MAC agar.

What is Pasteurella multocida?

500

Pseudomembranes in the intestines is a symptom of what type of infection?

What is Clostridium difficile?

500

Organisms that produce a black pigment after 5 days of growth on laked K-V blood agar

What is Prevotella or Porphyromonas?

500

An isolate that is non-hemolytic and requires NAD for growth.

What is Haemophilus parainfluenzae?

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