Mouse!
The genus and species of the house mouse (mouse used for research)
What is Mus musculus?
The locations of the eye wash station and fire extinguishers
What is:
by the wet lab sink - eyewash
by the front of the lab and by the fume hood - fire extinguishers
Bleach is effective for this long.
What is up to 1 week, but should be made fresh for hazards like viruses?
Cost of a 100-1000uL VWR pipettor.
What is $386.26?
This brain region is commonly abbreviated LHb.
What is the lateral habenula?
When picking up a mouse in a tail hold, you should hold them this far up from the base of the tail.
What is about 1/3 of the length of the tail from the base?
This is the first thing you should do if you spill something hazardous or injure yourself in lab.
What is notify a supervisor or PI immediately?
This surgery approach allows targeted delivery of virus, cannulas, fibers, or implants into specific brain regions.
What is stereotaxic surgery?
Cost of 20mg of fluoro-gold
$370
This brain region is commonly abbreviated DR or DRN and is often associated with serotonin.
What is the dorsal raphe nucleus?
This behavior can indicate pain, stress, or illness, and should be reported to a grad student or David
What is hunched posture, reduced movement, poor grooming or abnormal behavior?
This document tells you the hazards, PPE, storage requirements, and spill procedures for a chemical.
What is an SDS, or Safety Data Sheet?
This type of virus can be used to label neurons that project to a target region.
What is a retrograde virus or retrograde tracer?
Cost of 100ul of mCherry primary antibody (used in polysynaptic IHC)
What is $531?
This brain region is commonly abbreviated PBN and is important for sensory, pain, and aversive signaling.
What is the parabrachial nucleus?
This is why mice are usually handled gently and consistently before behavioral testing.
What is to reduce stress and variability in behavioral data
This is the minimum PPE for handling fentanyl.
You are asked to perform a procedure you have not been trained on. What should you do?
What is say that you have not been trained and ask for supervision?
Cost of the analytical balance in the wet lab.
What is $6,980.00?
DREADD
What is Designer Receptor Exclusively Activated by Designer Drug?
All experiments using animals need to be approved by this university agency.
What is IACUC (institutional animal care and use commitee)?
Megan tells you to do something involving a chemical you think is hazardous. You aren't comfortable handling the chemical. Megan continues to try and force you to complete a task with it, and starts yelling at you. You should...
What is leave the lab/walk away and talk to Dr. Barker?
also
What is do NOT handle the chemical?
Name all of the behavioral tests we are currently running for the stress susceptibility project.
What is:
Open field, Von Frey, Hargreaves, Sucrose Preference Test, Forced swim test
Cost of a refurbished version of our cryostat (i.e. discounted)
What is $24,500.00?
SharcQ
What is Slice Histology Alignment, Registration, and Cell Quantification?