Definitions
The definition of replicon.
All DNA replicated from a particular region
What does cDNA stand for?
Complementary DNA
The components of telomerase.
Protein & RNA
what enzymatic activities does beta polymerase have?
UNKNOWN
what kind of mutations are generally caused by intercalating agents?
Insertion or deletion
The definition of telomere.
The end of the eukaryotic chromosome
PCNA protein is structurally similar with what protein?
Sliding clamp/ β subuinit of Pol III holoenzyme
What kind of RNA can be destroyed by RNaseH?
The RNA strand in the RNA- DNA hybrid helix
what enzymatic activities does γ polymerase have?
- replicates mitochondrial genome
What kind of mutation can be caused by the incorporation of thymine analog 5-bromouracil?
t to c change
the definition of point mutation
alters a single nucleotide
The role of telomerase RNA?
template for adding the telomeric sequence to the 3' terminus end of the chromosome
human chromosome DNA sequence?
5'-TTAGGG-3'
what enzymatic activities does alpha polymerase have? (there's 4)
- Primase
- lacks exonuclease activity
- initiation of replication
- lagging strand synthesis
Deamination of cytosine changes C to which base and cause what kind of mutation after replication?
U, change from C:G to A:T (transition)
the definition of transition
pyrimidine to pyrimidine or purine-to-purine substitution (A to G, C to T)
The role of telomerase protein?
DNA pol activity extending the 3' end of the DNA substrate
Eukaryotic chromosome contains single or multiple replication origins?
multiple
what enzymatic activities does δ polymerase have?
- lacks primase activity
- 3' to 5' exonuclease activity
- infinite processivity with a PCNA (proliferating cell nuclear antigen protein)
- lagging strand synthesis
What kind of mutation can be caused by alkylation at the oxygen atom of guanine?
Mispairs with thymine, changing the G:C base pair to a A:T base pair during DNA replication.
the definition of transversion
Pyrimidine-to-purine or purine to pyrimidine (A to C or T to G/A)
What is the template of reverse transcriptase?
RNA
Which protein is responsible to detect the distortion in DNA caused by mismatches?
MutS
what enzymatic activities does epsilon polymerase have?
- high processivity in the absence of PCNA
- 3' to 5' exonuclease activity
- synthesis of leading strand
- repair UV- induced DNA lesion
The mutations that can be directly removed and the mechanisms involved.
1. O^6-methylguanine
2. methyltransferase
3. UV irradiation
4. DNA photolyase