Let’s talk about genes
The process of copying a segment of DNA into RNA
What is transcription
Sequencing of all RNA in a cell
What is RNA-seq?
This will tell us where each read came from in the genome.
What is mapping?
This plot which helps show over and under expression of genes resembles a fiery eruption.
What is a volcano plot?
This python data structure shares its name with cuddly black and white animals.
What is pandas?
Process that allows one gene to code for multiple proteins
What is splicing?
Sequencing of all DNA in a cell.
What is DNA-seq?
Technique to correct for technical variation across samples in gene expression data.
What is normalization?
Name of your mini project in the spring.
What is ….
Statistical test to compare means of two populations
What is a t-test?
Series of steps or reactions that produce a product or cause an outcome, often controlled by genes.
What is a pathway?
Technology that revolutionized sequencing by making it possible to generate billions of reads quickly and cheaply.
What is NGS/massively parallel sequencing?
Name of your mentor in the spring
who is ….
This plot represents gene expression values by a range of colors.
What is a heatmap?
This animal features heavily in BDiB stream photos.
What is a walrus?
Segment of DNA that codes for protein
What is an exon?
What is a fastq file?
Analysis to identify overexpressed and under expressed genes across conditions.
What is differential expression analysis/hypothesis testing?
A dimensionality reduction technique that plots samples on axes representing biggest sources of variation in the data.
What is PCA?
Groups of genes that show similar expression patterns across samples.
What are co-expressed genes?
These proteins bind to DNA in order to control the rate of conversion of a DNA template into RNA.
What is a transcription factor?
The average number of reads that cover the genome or transcriptome reference.
What is coverage/sequencing depth?
Metric used to indicate a gene is over or under expressed in one condition over the other.
What is fold change/log 2 fold change/p value?
Grouping data such that objects in the same group are more similar to each other that to objects in other groups.
What is data clustering?
The assumption that a gene’s expression is not affected by the condition/sample.
What is the null hypothesis?