Name the allosteric inducer molecule that binds to the lac repressor.
What is allolactose?
100
For translation to occur properly in prokaryotes, each gene must have a ____ and ____.
What is a ribosome binding site (RBS) and stop codon?
100
The two domains of SR proteins are ____ and ____.
What are RNA-binding domain and RS domain?
100
This protein binds the 5’ cap and helps to initiate translation.
What is eIF4E (CAP binding protein)?
100
This assay uses antibodies to detect proteins on a blot.
What is a western blot?
200
AraC is a Repressor/Activator/both (pick one).
What is both?
200
This type of protein binds after HAT proteins act on a histone.
What are bromodomain proteins?
200
This allosteric protein is bound to the iron response element, stabilizing the repression loop and inhibiting translation, in the absence of iron.
What is Iron Regulatory Protein (IRP)?
200
This type of mutational analysis test could be performed to provide evidence of a direct interaction between two molecules.
What is second-site suppression mutation analysis?
300
This protein when bound by cAMP activates transcription of the PBAD promoter in the absence of glucose.
What is CAP?
300
Of the two things S7 protein can bind to, name the one it has the highest affinity for this.
What is 16S rRNA?
300
Transcriptional silencing of one chromosome in females is known as _(blank)_.
What is X-inactivation?
300
This protein binds the polyA tail and eIF4G to help recruit 40S ribosomes.
What is polyA binding protein (PABP)?
300
This technique helps identify how much RNA is being actively translated.
What is sucrose gradient sedimentation?
400
The two segments of the leader region of the trp operon that bind to each other in the presence of high levels of tryptophan.
What are regions 3 and 4?
400
The initiator tRNA binds the P site, followed by 50S. The initiator tRNA is charged with an ______.
What is N‐formyl methionine (instead of a methionine)?
400
Although it is not sequence specific, this cis site (_____) is the only site flexible enough to allow for this protein (_____) to bind and bend the DNA 80 degrees.
What is AT-rich sites bound by TATA Binding Protein (TBP)?
400
This protein functions as a helicase to unwind any secondary structures that have formed in the mRNA.
What is eIF4A?
400
How the Sanger method was improved.
What is by placing fluorescent labels on the dideoxy-nucleotides?
500
This DNA region, found between sigma associating regions, is not conserved in organisms, though provides proper spacing for the binding of RNA pol.
What is the DNA between the -35 and -10 regions?
500
This eukaryotic protein interacts with RNA Polymerase II in a similar fashion to the prokaryotic σ 3/4 linker, and helps determine transcription direction by creating asymmetry in the pre-initiation complex.
What is TFIIB?
500
Name the location of the cis-element Iron Response Element (IRE) must be in order for translational repression to occur.
What is the 5’ end of the ferritin mRNA?
500
This biochemistry test can validate whether two proteins are interacting.