Genetics 101
Lambda phage
Prokaryotes do "it" this way
Eukaryotes transcribe this way
Technique This Round 2!
100
This mutation results in a complete loss of gene product and is typically recessive.
What is an amorph (or null) mutant?
100
This transcription factor is important for induction of the lytic cycle in lambda phage.
What is cro?
100
Prokaryotes have a ____ (higher/lower) level of gene density compared to eukaryotes.
What is higher?
100
Splicing silencers that bind to exon splicing enhancers (ESE) and block splicing because _____.
What is they lack the RS domain that recruits U1 & U2AF.
100
This method is used to separate macromolecules.
What is gel electrophoresis?
200
What does dyad symmetry in DNA suggest about the protein’s structure that binds to that particular DNA sequence?
What may be a homodimer (or homomultimer)?
200
If there is more than one lambda phage that infects a single E. coli cell, the viral genome that ends up infecting the cell will likely express this pathway.
What is Lysogenic?
200
This part of the sigma factor mimics RNA and blocks the exit channel.
What is the 3/4 linker?
200
Tup1 is a co-activator/co-repressor and binds via protein:DNA/ protein:protein interactions.
What is a co-repressor and a protein:protein interaction?
200
This assay uses a sequence specific radioactive probe to detect DNA presence.
What is a Southern blot?
300
This mutation is dependent on environmental factors.
What is a conditional (or temperature sensitive) mutant?
300
Lambda repressor has the highest binding affinity for BLANK, while Cro has the highest binding affinity for BLANK.
What is OR1 for lambda repressor and OR3 for Cro?
300
AraC uses this mechanism to repress its own transcription.
What is autoregulation?
300
Name the consensus sequence that is usually found at the 5' splice site of an intron.
What is GT?
300
This assay is ran on a non-denaturing electrophoretic gel to determine whether proteins are bound to DNA or RNA.
What is mobility shift (EMSA)?
400
This mutation in the Lac repressor results in an uninducible phenotype.
What is a LacI^S mutant?
400
If CII and CIII are both mutated, plaques will appear more (turbid or clear).
What is clear?
400
A transposable element that contains only one gene encoding for its own transposase.
What is the IS element (Insertion Sequence element)?
400
An insulator should be placed here in order to limit the functional distance of an enhancer.
What is between the enhancer and neighboring gene(s) that should not be expressed?
400
This assay is used to detect gene expression levels with the use of a sequence specific radioactive probe.
What is a northern blot?
500
Tumor cells with these mutations are usually recessive, and require two defective copies to promote cancer cell formation.
What are tumor suppressor genes?
500
These three form the complex that allows N anti-termination in the lambda phage genome.
What are the RNApol, Nus proteins, and N proteins?
500
Double JEOPARDY! Sigma must dissociate for elongation of transcription to begin in prokaryotes. List the four components that must be displaced and why each component is significant.
What is - Region 2 binds to -10 region -Region 4 binds to -35 region by helix turn helix -3/4 linker blocks RNA exit channel -1.1 subunit blocks template strand in open complex
500
Genomic Imprinting works on this kind of chromosome.
What is an Autosome?
500
This assay takes advantage of the modularity of proteins and tests whether 2 proteins interact by fusing each protein to either a DNA binding domain or to an activation domain of a protein such as GAL4. If the proteins interact, then RNApol II can be recruited so transcription and thus translation can occur.
What is a yeast two-hybrid?
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