Passing boi-ology (H.S. level for 1st years)
This organelle is considered a "powerhouse", one capable of providing all energy needs for the cell
Mitochondria
What is the central dogma of molecular biology
DNA -> RNA -> Protein
What is the programming language most commonly used in bioinformatics?
Python
Which biomolecules does bioinformatics sequence and what is the preparation of it called?
DNA and RNA, it is called "Library Prep"
This eclectic scientist was one of the first to study microorganisms using a microscope, naming them "cells" meaning "little room" in latin after the honeycombs he was reminded of
Who is Robert Hooke?
What is Chargaff's rule?
% purines = % pyrimidines
What programming language is statistical analysis of biological data done in?
R
How can we sequence RNA?
Through reverse transcription to create cDNA which can then be sequenced
LUCA > last universal common ancestor
Which amino acid has a sulfur atom and forms disulfide bonds
Cysteine
Once we have a raw DNA sequence file we use code on it, what is the code for?
to refine the data
What is the most common technology used to prepare Library Prep of DNA?
This process converts mRNA to protein
Translation
This gene-editing system makes use of a bacterial immune response protein originally designed for defense against phages
What is CRISPR (clustered regularly interspaced palindromic repeats)?
T/F: we can only do code on DNA sequences and NOT on RNA sequences and Protein sequences
FALSE
What are some applications of Bioinformatics (give at least 2 to get a full point, it can be any two)
- evolution analysis
- cancer > finding driver mutations
- genetic modifications
- find disease-causing mutations
- gene therapy
Cell Theory has these three main principles (hint: the last refutes abiogenesis or the concept of life arising from nothing)
1) All organisms are composed of cells
2) Cells are the basic unit of life
3) All cells come from other cells (non-spontaneous generation)
This protein involved in PCR was isolated from the hot springs of Yellowstone National park
I need to create a script that will process a sequence of DNA, what platform can I use to create this script and code it?
Linux / Terminal
I have a dysfunctional protein in a cell. I want to see if the cause is a mutation in the gene(s) encoding the protein. What type of sequencing should I do?
Whole Exome Sequencing > proteins made up by amino acids which are translated from exomes in the DNA molecules