Microbial Growth 1
Microbial Growth 2
Microbial Control
Microbial Control 2
100
What are biofilms?
Biofilms are cities of microbes - where they grow!
100
What are the four environmental components that have an impact on microbial growth?
Temperature, pH levels, amount of Oxygen, amount of salt concentration.
100
What is decontamination?
Decontamination is using chemical, physical, and mechanical methods to destroy or reduce undesired microbes, particularly those that cause infection.
100
Radiation: Ionizing vs. NonIonizing Radiation
Ionizing: short wavelength, high energy. For medical supplies and vaccines. Nonionizing: sporicidal, but non penetrating. Mainly for surfaces.
200
What is binary fission?
Binary fission is the replication of the cell to form two cells.
200
How large can bacterial populations grow in rich media? (per mL)
10^8 to 10^9 cells per mL
200
Disinfection vs. sterilization vs. antisepsis vs. antibiotics What they target, if they kill or just reduce, and where they are applied.
Disinfection - destruction or removal of vegetative pathogens, but not endospores. (inanimate objects) Sterilization - kills everything (inanimate objects) Antisepsis - inhibits or destroys vegetative pathogens, usually on the skin Antibiotics - targets specific pathogens inside the body
200
Give an example of mechanical control?
Filtration, such as filtrating air in hospital rooms or in liquids.
300
Define the following bacterial shapes: baciullus, coccus, vibrio, spirilium, spirochete
Baciullus - rod Coccus - circle Vibrio - comma shaped Spirilium - spirally Spirochete - extremely spirally, corkskrew
300
WTF is a biofilm
Biofilms are how bacteria grows in nature.
300
What are the three major classes of control?
Mechanical, physical, and chemical.
300
What is chemical control? What are the levels?
Low level, medium/intermediate, high level.
400
How is bacterial growth measured? Name at least two ways.
Number of cells, changes in turbidity, count in a counting chamber.
400
What are the three phases of a biofilm life cycle.
Attatchment, growth, and detatchment.
400
How can you tell that microbes are dead? At least 2 ways.
They stop reproducing, the numbers of them, the agent used, the environment.
500
What are the four phases of microbial growth? Explain a little about each one.
Lag, exponential, stationary, death.
500
Explain the attatchment, detatchment, and growth phases of the biofilm life cycle.
Attatchment: the cells attatch to a surface of protein. Growth: growing Detatchment: bacteria are released into the bloodstream and cause disease.
500
What are some methods of physical control? Give at least two methods. +50 for each extra.
Heat (moist + dry: autoclaves and ovens), cold, desiccation, radiation .
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