Pipettes
Micropipette size = P10
Volume measured = ?
Volume measured = 0.5-10 ul
What are two benefits of the negative stain?
1. The bacteria do not shrink from the heat
2. Hydrophobic microbes can be visualized with this technique
1ul = ? ml
1 ul = 0.001 ml
What is the name of microbes that can tolerate very high sugar concentration?
Osmophiles
Micropipette size P20
Volume measured?
2-20ul
How does the dye we use in a negative stain work?
Nigrosine carries a negative charge which is repelled by the negative charge of bacterial membranes (teichoic acid and lipopolysaccharides)
100ul = ? ml
100ul = 0.1 ml
What is the name of microbes that require oxygen to grow but cannot tolerate high concentrations of
oxygen?
Microaerophilic
Micropipette size P200
Volume measured?
20-200ul
What is the term used when a variety of shapes can be seen in a sample?
pleomorphism
1.5 ml = ? ul
1.5 ml = 1500 ul
The highest temperature at which an organism can grow is called the _______.
The lowest temperature at which an organism can grow is called the ______.
The ideal temperature at which an organism can grow is called the _______.
Maximum
Minimum
Optimum
Micropipette size = P1000
Volume measured = ?
100-1000ul
What does the acid-fast stain test for?
Differential stain that detects the presence or absence of mycolic acids in the bacterial cell wall
What are mycolic acids?
Waxy materials composed of fatty acids and fatty alcohols and are found in the cell walls of bacteria from genera, Mycobacterium and Nocardia