Enzymes & Kinetics
Lipids & Membranes
Transcription
Nucleotides & Nucleic Acids
DNA Replication
100
A catalytically active complete enzyme together with its bound coenzyme and/or metal ions
What is a Holoenzyme?
100
These are impermeable to most polar/charged solutes and permeable to non-polar compounds
What is a membrane?
100
The DNA strand that is the template for RNA – it is complementary to the RNA
What is antisense?
100
undergo extensive modifications after synthesis by RNA
What is Eukaryotic mRNA
200
A catalyst that changes the rate of a chemical reaction but does not alter the equilibrium.
What is an enzyme
200
translocate primarily aminophospholipids (PE & PS) from outer to inner leaflet & using ATP
What is flippase
200
Upstream of initiation
What is TATA box
200
Histones bind to DNA to form these.
What is a nucleosome
200
used by ribosomes to mark the beginning of the Translation sequence
What is The Shine-Delgarno sequence
300
The protein part of such an enzyme.
What is a Apoenzyme (aproprotein)
300
measured by Fluorescence Recovery after Photobleaching (FRAP)
What is lateral diffusion?
300
Synthesized at a constant rate. Typically involved in basic cellular functions
What is Constitutive Enzymes
300
Might be living fossils. From much earlier times when RNA was genetic material and the catalyst for its replication. Significant due to self replication.
What is the RNA world hypothosis
300
Deoxyadenylate is an example of what
What is a nucleotide
400
A coenzyme or metal ion that is covalently or very tightly bound to the protein
What is a Prosthetic group?
400
utilizes a lipid moiety to anchor the protein to the lipid bilayer
What is a type V membrane
400
Terminates Transcription
What is the rho factor?
400
In all cellular DNAs, irrespective of species, the # of adenine residues equals the # of thymidine residues and the # of guanine residues equals the # of cytosine residues. i.e. A = T & G = C therefore A + G = T + C (the sum of the purines equals the sum of the pyrimidines)
What is Chargaff’s Rules?
400
uses DNA glycosylases to remove a damaged adenine, thymine, cytosine, or guanine base
What is Base Excision Repair
500
The theory that a catalyst functions by lowering the activation energy of a reaction, the energy barrier for the reactants to become products
What is the transition state theory?
500
Clathrin, COPI, and COPII are this type of vesicle
What is a coated vesicle
500
Synthesizes mRNA precursors, located in nucleoplasm
What is RNAP II
500
Successfully used in many loss-of-function studies in eukaryotes by suppressing gene expression post-transcriptionally
What is RNAi
500
Found on the lagging strand that are joined by DNA ligase
What is Okazaki fragments
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