Lab Safety
Pipetting
Spectrometry
Enzymes
Hypotheses and Predictions
100

Type of footwear that should be worn in lab.

What is closed toed shoes with no holes.

100

Used to transfer small, set volumes of liquid.

What is a transfer pipette?

100

Arrangement of a spectrometer.

What is a white light source, cuvette, detector, and display?

100

Place where substrates fit into the enzyme.

What is the active site?

100

Null hypothesis.

What is independent variable will not have a significant effect on dependent variable?

200

Gloves should be disposed here.

What is a biohazard bag?

200

Gradations go all the way down to the tip.

What is a serological pipette?
200

Used to convert absorbance values to concentration.

What is a standard curve equation?

200

How enzymes speed up a reaction.

What is lowering the activation energy?

200

Alternative hypothesis.

What is independent variable will have a significant effect on dependent variable?

300
Broken glass should be disposed of here.

What is glass disposal container?

300

A P10 with a readout of [0|2|5]

What is 2.5 microliters?

300

The raw value obtained from the spectrometer.

What is absorbance in ODU?

300

Catechol turns into this substance after it interacts with catecholase.

What is benzoquinone, a brown product?

300

Includes direction.

What is a prediction?

400

Clean-up procedures.

What is wiping your bench down and returning all materials to their proper space?

400

A P1000 pipette with a readout of [1|0|0]

What is 1000 microliters? (1 mL)

400

Absorbance is ________ to concentration.

What is directly proportional?

400
Temperatures that are too high can do this to an enzyme.

What is denature?

400

Does not include direction.

What is null and alternative hypotheses?

500

Laboratory Safety Equipment in the Lab.

What are Eyewash, Fire Extinguisher, First Aid Kit, Safety Glasses, and Gloves?

500

A P1000 pipette with a readout of [0|7|1]

What is 710 microliters?
500
Wavelengths that are the color of the object.

What are the transmitted wavelenghts?

500

Typically, higher substrate concentration does this to a reaction.

What is increases the rate and products formed?

500

Prediction.

An (increase/decrease) in the independent variable will (increase/decrease) the dependent variable.

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