What is enucleate egg, fuse with somatic cell, culture for a few days, into surrogate mother
100
Aim of gene therapy
What is add in correct gene so correct protein is made and correct function is allowed
100
Define genome
What is all genes in an organism
200
3 components of a nucleotide?
What is sugar, base, phosphate
200
3 differences between DNA and RNA
What is double, single stranded, deoxyribose, ribose, T, U
200
Aim of reproductive cloning?
What is new being
200
Example of disease that may be treated by gene therapy?
What is DMD, Sickle Cell, CF etc
200
Define genomics
What is the study of all genes in an organism
300
Complementary base pairs?
What is A, T.....C, G
300
Transcibe CGT GGA
What is GCA CCU
300
How do you clone therapeutically?
What is enucleate egg, fuse with somatic cell, culture for a few days, collect ES cells, culture and grow into desired cells
300
2 strategies of gene therapy?
What is in vivo and ex vivo
300
Define pharmacogenomics
What is study of effects of genes on an individuals response to drugs
400
Bases held together by what type of bonding?
What is hydrogen
400
Translate UUU GGU
What is Phe, Gly
400
Aim of therapeutic cloning?
What is genetic matched tissues for transplant
400
3 ways to deliver genes
What is viral vectors, naked DNA, liposomes
400
Promises of pharmacogenomics?
What is more effective drugs perscribed, better dosing, more effective drug development
500
Name used to describe strands running in opposite directions?
What is antiparallel
500
4 scenarios that can occur when a base substitution occurs
What is no change in amino acid, change in amino acid but no change in protein function, change in amino acid but change in protein function, introduction of stop codon
500
Why is therapeutic cloning controversial?
What is destruction of blastocyst
500
Concerns of gene therapy?
What is expression in right place, right time, viral vectors, immune responses to 'new' protein, what is expression shut down?
500
Define gene expresssion
What is gene switched on, RNA or protein made from the DNA, active