Elisa and Benedict's Test
How long, and at what temperature, are samples incubated during the Elisa test?
5 minutes at room temperature
Type I diabetes
What must organic molecules contain?
Carbon atoms covalently bonded to hydrogen atoms
What is the monomer?
Fatty acids
What type of sugars make up DNA?
What is the positive and negative results of the Benedict's and Elisa test?
Benedict's positive- red
Elisa positive- green
Benedict's negative- blue
Elisa negative- clear
Symptoms of diabetes
1:2:1
Are they hydrophobic or hydrophilic?
hydrophobic
What are the purines?
Adenine and guanine
What is the order of reagents added in the Elisa test?
- capture antibody solution
-wash
-patient sample
-wash
-detection antibody solution
-wash
-substrate solution
Normal blood sugar range
75-100 mg/dL
What is the bond called that is formed during dehydraytion synthesis?
What is the functional group in fatty acids?
carboxyl
What end do nucleotides have to be added onto? What is this bond called?
3' end
Phosphodiester linkages
What is the purpose of the substrate solution in the Elisa test?
There is an enzyme attached to the detection antibody. The substrate binds to the active site of the enzyme and catalyzes a reaction that produces the green color in positive samples.
Beta cells in pancreas
How many monomers are in a polysaccharide?
10+
What term describes a phospholipid (Think of the differences between the head and the tail!)
Amphipathic
What is the active site?
The place where the substrate binds to the enzyme
What does the Benedict's test look for? Why?
Glucose in the urine - glucosuria
Excess glucose cannot be excreted by kidneys in patients with diabetes
Type I - autoimmune disorder of the beta cells, cannot produce insulin
Type II- the body becomes resistant to insulin (even if insulin binds to receptors, the glucose transporter does not work) or does not produce enough, usually caused by environmental factors
Which are the complex carbohydrates?
oligosaccharides and polysaccharides
What bond forms during dehydration synthesis?
Ester linkage
What bond holds amino acids together?
Peptide bonds