Stain Alive
pHahrenheit 451
Salty and Air-ogant
yoUVe Got to Be Killing Me
The Fastidious and The Furious
100

Cell membranes carry this net charge

What is negative

100

Temperature at which a species experiences maximum cell division

What is optimum growth temperature

100

Aerotolerance class that does not grow in presence of oxygen

What are obligate anaerobes

100

This range of antibiotics would be prescribed if the pathogen has not yet been identified

What is broad-range

100

This agar is used to test for caseinase

What is skim milk agar

200

These are the four reagents used in Gram staining, in order

What is crystal violet, iodine, ethanol, and safranin

200

Term used for species which grow best at pH 5.5 or less

What is acidophile

200

When the concentration of solutes is the same both outside, and inside the cell

What is isotonic

200

This type of antimicrobial can be applied to living tissue

What is antiseptic

200

Sheep's blood agar, with an unknown mixture of biological molecules, is an example of this type of media

What is complex/undefined

300

Endospores are compact fortresses, protecting this most valuable asset

What is DNA

300

Temperature class of bacteria which can be found in volcanic vents

What is hyperthermophile

300

Term used to describe species which withstand high sugar conditions

What is osmotolerant

300
In the Kirby-Bauer disk diffusion assay, does a large zone of inhibition indicate sensitivity, or resistance to an antibiotic

What is sensitivity

300

This method of sterilization is best for heat-sensitive liquids

What is filtration

400

Acid-fast cell membranes are composed primarily of this molecule

What are mycolic acids

400

Span of temperatures which allow a species to perform cell division

What is growth range

400

An obligate aerobe prefer an environment with _____ oxidation/reduction potential (high or low)

What is high ORP

400

Optimum wavelength at which DNA absorbs UV light

What is 260-265nm

400

These are three molecules required for all life on Earth

What is water/carbon/nitrogen/phosphorus/trace elements/sulfur/vitamins
500

These are the three reagents used in acid-fast staining, in order

What is carbolfuchsin, acid alcohol, and methylene blue

500

Most bacterial species fall within this pH tolerance group

What are neutrophiles
500

When the concentration of solutes is higher inside the cell, than outside, causing cells to swell

What is hypotonic

500

This term is used to describe disinfectants which halt bacterial cell division

What is microbiostatic

500

These are the three key variables which make an autoclave an efficient tool for sterilization

What are pressure, steam (or water), and temperature

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