CRISPR
This system enables scientists to edit parts of an organisms genome
What is CRISPR Cas 9?
The technique used to analyze and identify an individual
What is gel electrophoresis
This is the name of the small circular piece of DNA that some bacteria contain.
What is a plasmid?
This technique is used to amplify a gene of interest.
What is polymerase chain reaction (PCR)?
The enzyme portion of the CRISPR system that makes the cuts to the DNA
This is the name of the enzyme that cuts DNA at specific recognition sequences.
What is a restriction enzyme?
DNA produced by combining DNA from different sources
What is recombinant DNA?
In gel electrophoresis, DNA migrates towards this pole of the gel chamber.
What is the positive end (aka anode)?
What is the guide RNA?
This type of DNA analysis involves looking at a special type of polymorphism.
What is STR analysis (DNA fingerprinting)?
This process is when a bacterial cell takes on a recombinant plasmid.
What is transformation?
This substance is used as a medium for DNA to migrate and be separated during gel electrophoresis.
What is agarose gel?
What are bacteria/archaea?
It affects how fast the DNA strands move through the gel
What is the length of the DNA strands
This term refers to the overhangs formed after the DNA is cut by a restriction enzyme.
What are sticky ends?
An organism with genetic material from another organism.
What is Genetic Modified Organism (GMO)?
This scientist pioneered the use of CRISPR technology in her research
Who is Jennifer Doudna?
The filter that sorts the DNA strands according to their length
What is a Gel
This method refers to subjecting a bacterial cell to high temperatures to induce openings in the plasma membrane.
What is heat shock?
These organisms are modified with extra genes from their own species
What are cisgenic GMOs