Micropipettes
What a micropipette is used for
Accurately measure and use small amounts of liquid
Most important rule to remember when using a centrifuge
Balance
What happens to a DNA molecule after once cycle of PCR
It doubles
What kind of charge DNA carries
Negative charge
What "D" and "U" stand for
Digested and Undigested
Always use a ___ when using a micropipette
Tip
What a centrifuge is used for
Spin and mix small amounts of liquid
PCR unabbreviated
Polymerase Chain Reaction
DNA molecules that move the quickest
The smallest ones
Solution that was used to extract your DNA
Saline Solution
P200 measures between ___ and ___ microliters
20 and 200
How to know if a centrifuge is properly balanced
Each tube must have one tube directly opposite
How PCR differs from DNA replication
PCR is a lab procedure, DNA replication occurs naturally
How many wells were in the gels we cast
9
Liquid that was poured on gels
Buffer
What measurement the numbers 134 would represent on a P20
13.4 microliters
___ is used to balance a centrifuge with an uneven number of tubes
Balance tube or blank tube
The three steps that make up PCR
Denaturation, Annealing, Extension
The gene/trait we were examining in this lab
Ability to taste PTC
Restriction enzyme used to digest your DNA
Haelll
What µL represents
Microliter
How many tubes our centrifuge can spin at once
8
The four bases that make up DNA
adenine, thymine, cytosine, guanine
How many bands you should have seen (per lane) in your final results
1, 2, or 3
How many cycles of PCR were done in one sitting (about 45 minutes)
30