Enzymes
DNA Manipulation
Genetic Engineering
Applications of Biotechnology
Ethical Considerations
100

This type of enzyme recognizes specific DNA sequences and cuts the DNA at or near these sites.

What is a restriction enzyme?

100

The process of removing DNA from a cell to study or manipulate it.

What is DNA extraction?

100

This process involves making changes to an organism’s DNA for practical purposes.

What is genetic engineering?

100

The medical procedure that aims to replace faulty genes with functional ones.

What is gene therapy?

100

A potential benefit of GMOs.

What is reducing pesticide use, increasing crop yield, or improving nutrition?

200

A restriction enzyme recognizes this type of sequence, which reads the same forward and backward.

What is a palindrome?

200

DNA fragments move through a gel from this charge to this charge.

What is negative to positive?

200

A small, circular piece of DNA found in bacteria that is often used in genetic engineering.

What is a plasmid?

200

This process involves choosing organisms with desirable traits to reproduce, gradually enhancing those traits over generations.

What is selective breeding?

200

A potential risk of GMOs.

What is the possibility of allergic reactions, loss of biodiversity, overuse of herbicides, or unintended crossbreeding?

300

This technique is used to separate DNA fragments after they have been cut by restriction enzymes.

What is gel electrophoresis?

300

The purpose of this biotechnology technique is to separate and analyze DNA fragments based on size.

What is gel electrophoresis?

300

Combining DNA from two or more different sources creates this type of DNA.

Combining DNA from two or more different sources creates this type of DNA.

300

The difference between reproductive and therapeutic cloning.

What is reproductive cloning creates whole organisms, while therapeutic cloning creates stem cells for medical treatment?

300

One ethical concern about cloning humans.

What is reduced genetic diversity, loss of individuality, or potential misuse?

400

Two reasons why Gel Electrophoresis would be performed.

What is forensics, paternity tests, gene analysis, study the structure and function of proteins?

400

Before scientists can analyze DNA, they must make many copies of a specific DNA segment using this technique.

What is polymerase chain reaction (PCR)?

400

Scientists use this process to create genetically identical copies of organisms or cells.

What is cloning?

400

One goal of genetically modifying crops.

What is increasing yield, improving resistance to pests, or enhancing nutritional value?

400

A potential risk of gene therapy.

What is unwanted immune system reaction? Targeting the wrong cells? Infection caused by the virus? Possibility of causing a tumor?

500

This unique pattern of DNA bands, created through gel electrophoresis, can be used to identify individuals.

What is a DNA fingerprint?

500

A genetically modified organism that contains DNA from a different species.

What is a transgenic organism?

500

One major concern with this process is that it reduces genetic diversity, making populations more vulnerable to disease and environmental changes.

What is cloning?

500

An example of an modern organism produced from selective breeding.

What is corn (or many other fruits and vegetables)? What are dogs? What are cows that produce higher yields of milk?
500

A key ethical question regarding gene therapy.

What is killing embryos in the process?

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