PCR Basics
Polymerase Chain Reaction
What does PCR stands for?
Restriction Fragment Length Polymorphism
What does RFLP stand for?
It creates unique DNA profiles for comparison.
How does RFLP help in DNA analysis for crimes?
Denaturation, Annealing, and Extension
Name the three steps in PCR.
Restriction enzymes
Which molecular tool is used to cut DNA into fragments in RFLP analysis?
Contamination or degradation of DNA samples.
What challenge might affect DNA evidence reliability in investigations?
DNA Polymerase
Which enzyme is used in PCR to synthesized new DNA strands?
Genetic variations.
In RFLP analysis, what does the length of DNA fragments reveal?
RFLP (Restriction Fragment Length Polymorphism)
What method creates unique DNA patterns used in crime investigations?
To separate the DNA double strands.
What is the purpose of the denaturation step in PCR?
Gel Electrophoresis
Which process separates DNA fragments in RFLP analysis?
It copies DNA for studying genes or making copies.
What does PCR do in genetic research?
About 30 cycles.
How many cycles of denaturation, annealing, and extension typically occur in a PCR process?
Southern blotting
What process involves transferring DNA fragments from a gel to a membrane for analysis in RFLP?
It detects specific germs by amplifying their DNA.
How does PCR help in diagnosing diseases?