Percent Solutions
Dilutions
Conversions
Standard Curve
Sig Figs
100

Prepare a 500 mL of 10% bleach solution (v/v)

What is 50 mL?

100

What is the formula when solving stock solution type questions?

What is C1V1= C2V2?
100

0.75 L = _______ mL

What is 750?

100

What is the purpose of creating a standard curve?

What is it allows you to determine the concentration of an unknown solution?

100

0.00340

What is 3?
200

Prepare a 50 mL of 1.5% agarose in buffer (w/v).

What is 0.75 g?

200

A technician needs to prepare 350 mL of 1X TAE using a 50X TAE stock solution. Calculate the volume in mL

What is 7 mL?

200

450 µg = _______ mg

What is 0.45mg?
200

A solution has an absorbance of 0.8 at a concentration of 0.3 M. What would the absorbance be if the concentration is tripled?

What is 2.4?

200

7000

What is 1?

300

A culture medium recipe calls for 2% glucose. If you are preparing 500 mL, how many grams of glucose are required?

What is 10g?

300

A technician needs to prepare a 500 mL of 0.25 M sucrose using a 1 M stock solution. Calculate the volume in mL.

What is 125 mL?

300

0.006 kg = _______ g

What is 6?

300

A protein solution has an absorbance of 1.2 at a concentration of 0.5 mg/mL. If the concentration is reduced to half, what will be the new absorbance?

What is 0.6?

300

1.000 × 10³

What is 4?

400

Determine the mass (in g) of NaOH that you would need to create 1000 mL of a 0.01M solution. (molar mass = 39.99 g/mol). Round to two decimal places.

What is 0.04?

400

What is the dilution factor if you add 250 uL of protease standard solution to 250 uL of buffer?

What is 2 or 1:2?

400

A lab protocol calls for 25 µL of plasmid DNA per sample. You have 12 samples. How many mL of plasmid DNA will you need in total?

What is 0.3 mL?

400

If a sample has an absorbance of 0.62 AU, using the formula below, what is the protein concentration (mg/mL)? **Round to 2 decimals 

 y= 2.1803x + 0.098.

What is 0.24 mg/mL?

400

The concentration of an enzyme solution is calculated as 0.0045678 M but should be reported with 3 significant figures. What should the final reported value be?

What is 0.00457 M?

500

A PCR reaction requires 0.2 mL of 50% glycerol solution in a 1 mL final reaction. What is the % of glycerol in the final reaction? 

What is 10%?

500

A technician needs to prepare a 200 mL of 0.1µg/mL plasmid using a 10 mg/mL stock solution. Calculate the volume in mL

What is 0.002 mL?

500

Convert 5 mg/min to g/hr

What is 0.3 g/hr?

500

What is the protein concentration (mg/mL), using the formula below for a sample reading of 0.25? ** Round to 1 decimal point

y= 0.0014x + 0.0086

What is 172.4 mg/mL?

500

456.2 − 0.03

What is 456.2?

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