This links fragments of DNA back together in recombinant DNA technology.
What is DNA ligase?
100
These DNA fragments move more quickly through the gel.
What is smaller?
100
This enzyme is utilized in PCR
What is taq polymerase?
100
DNA transformation involves the transfer of DNA via
What is a plasmid?
200
This school supply is an analogy for the restriction enzyme.
What are scissors?
200
This is a circular piece of DNA found in bacteria.
What is a plasmid?
200
This Jell-O like substance acts as a molecular strainer.
What is agarose gel?
200
This is the full name of the process, PCR
What is polymerase chain reaction?
200
When DNA from an ampicillin resistant organism is brought into an ampicillin sensitive organism by transformation and the product is grown on agar containing ampicillin
bacteria will grow
300
This term refers to the overhangs formed after the DNA is cut by a restriction enzyme.
What are sticky ends?
300
This process is when a bacterial cell takes on a recombinant plasmid.
What is transformation?
300
Adding these at the end of electrophoresis radioactively labels DNA fragments.
What are probes?
300
This is the order of steps in a PCR
What is denaturing, annealing, extending?
300
T or F: In transformation, both strands of donor DNA enter the recipient cell.
True
400
A term that means "carrier." A plasmid is an example of this term.
What is a vector?
400
This method refers to subjecting a bacterial cell to high temperatures to induce openings in the plasma membrane.
What is heat shock?
400
The charge of the end which DNA migrates toward during electrophoresis.
What is positive?
400
During this step, primers bind to the separated DNA fragments.
What is annealing?
400
This procedure served to open up the plasma membrane so that the plasmid could be inserted
What is heatshock?
500
A scientist uses EcoRI on DNA from a glowing organism, Vibrio fischeri. The scientist accidentally grabs the wrong bottle and adds HindIII (another restriction enzyme) to the plasmid. This percentage of plasmids will have the gene from the Vibrio fischeri in it.
What is zero?
500
This process refers to making many identical copies of bacteria that contain the new recombinant plasmid.
What is cloning?
500
The DNA moves towards the positive electrode because of the negative charge of this part of the DNA structure.
What is the sugar-phosphate backbone?
500
This is the source of the enzyme used during the extension phase of PCR
What is bacteria from hydrothermal vents?
500
This served as a control in the bacterial transformation (pGLO)