Amino Acids
Protein Structures
Membranes
Enzymes
Wild
100
What 3 groups are bonded to the alpha carbon?

Carboxyl, amino, R group

100

T/F: Fibrous proteins have several types of 2° structures.

False

100

T/F: Lipids are insoluble in H2O

True

100

What are the 3 reversible inhibition?

competitive, uncompetitive, mixed inhibitors

100

What is the equilibrium constant for weak acids?

Ka

200

What is the one letter symbol for asparagine?

N

200

2° structure is determined by...

backbone interactions

200

How many double bonds are in 20:5(Δ5,8,11,14,17) Eicosapentaenoic acid (EPA)?

5

200

When looking at a Michaelis-Menten graph, what does Km tell us?

Km = [S] @ 1/2 of Vmax

200

How does Van der Waals interactions work?

Happens when 2 uncharged atoms are very close and a transient dipole induces in opposite dipole in the nearby atom
300

T/F: The following amino acids R, Y, P, and I all contain at least 4 or more carbons in their side chains

False
300

What is a motif or fold?

recognizable folding pattern involving 2 or more elements of 2°structures and their components

300

What 3 classes of lipid membranes were discussed in class?

glycerophospholipids, sphingolipids, sterols

300

How do you compare enzymes?

specificity constant

300
What protein separation technique involves all proteins having a negative charge?

SDS Page

400

Polypeptide containing 1 Glu, 1 Ala, 1 Arg, and 1 Trp. Treatment of chymotrypsin (Trp (C)) led to a release of free Trp. This tells me...

Trp is in the 1st position

400

Chaperones may aid a polypeptide chain to adopt its native confirmation by...

binding to hydrophobic regions and preventing aggregation

400

How would you remove a peripheral protein?

Disrupt electrostatic or H bonds

400

How does a mixed inhibitor affects Km and Vmax?

Km is unchanged

Vmax app decreases

400

What are the 3 mechanisms of catalysis we learned in class?

general acid/base

covalent

metal ion

500

What is the net charge of aspartic acid at pH=4.5?

pKa amino group = 9.8

pKa carboxyl group = 2.1

pKa R group = 3.9

-0.8

500

What is the molecular reason for why detergents may cause a protein to denature?

Disrupts hydrophobic regions

500

Adding many molecules of phosphatidic acid to an aqueous environment would lead to the formation of a stable ____.

Bilayer

500

Km = 15 mM

Vmax = 0.005 mM/min

What would the initial velocity be if [S]=5mM in µM/min?


1.25 µM/min

500

Why is hemoglobin considered an allosteric protein?

The binding of one oxygen affects other oxygen binding sites.

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