Lab Safety & Experimental Design
The variable typically found on the Y-AXIS in figures or data visualization
What is the dependent variable?
The property of an enzyme that determines its function
What is an enzyme's shape?
The type of fermentation studied in the BISC 220 fermentation experiment
What is alcoholic fermentation?
The type of antigens present on a human blood cell of type A+ blood
What are A and Rh factor antigens?
The two properties that determine the movement and separation of the DNA fragments in gel electrophoresis
What are size and charge? (Charge makes the DNA move, size causes the separation)
The place to dispose of a new slide dropped on the floor
What is the clean sharps box (white cardboard box)?
The type of macromolecule tested for with Biuret's reagent
What is protein?
The conditions under which cells typically use fermentation
What is anaerobic OR low oxygen?
The tonicity of a solution that causes water to rush INTO an animal cell
What is hypotonic?
The size (general, not a number) of DNA fragments that will move more quickly through the gel during gel electrophoresis
What is small(er)?
The place to dispose of your chromatography solvent waste
What is a liquid chemical waste container?
The type of macromolecule that will test NEGATIVE for Benedict's test but POSITIVE for Lugol's reagent?
What is starch?
The metric by which we measure solubility of something in the working solvent using paper chromatography
What is Rf OR retardation factor?
The tonicity of a solution that has less dissolved solute than an animal cell within it
What is hypotonic?
The number of DNA fragments formed using a restriction enzyme that cuts in three (3) places on a plasmid
What is three?
The type of control described by the current drug/treatment in a study testing a new drug/treatment
What is a positive control?
The type of macromolecule that will test NEGATIVE for a Benedict's test without an HCl step but will test POSITIVE for a Benedict's test with an HCl step
What is a disaccharide/non-reducing sugar? (Sucrose is an example but is not the type)
The term for where you painted the spinach juice on the chromatography paper in your photosynthesis lab
What is the pigment origin (line)?
The type of antibodies produced by a human with type O+ blood
What are anti-A and anti-B antibodies?
The charge of the electrode you should put your wells CLOSER to in gel electrophoresis
What is negative? (The DNA will move towards the positive end, so the wells should be closer to the negative end)
The place to dispose of a micropipette tip that has been used to pipette BSA (bovine serum albumin)
What is the big red biohazard bin OR the small tabletop biohazard bin? -100 if you said either of the sharps containers
The sugar that reacted with Benedict's reagent to form red precipitate after an HCl step in our experiments
What is glucose? (Remember that the HCl cleaves sucrose so that its monosaccharides can react with Benedict's reagent; it is the monosaccharides/reducing sugars that actually help form the precipitate)
The formula for the metric by which we measure solubility of something in the working solvent using paper chromatography
What is
distance traveled by solute/distance traveled by solvent?
The two types of transport across a cell membrane that do NOT require energy input
What are diffusion and facilitated diffusion?
The parent you should compare the offspring's DNA bands to FIRST when doing a paternity test using gel electrophoresis
What is the mother? (Doing this allows you to determine which bands were inherited from the maternal side, so the rest of the bands will have been inherited from the paternal side)