ABO
The H gene elicits the production of a glycosyltransferase that transfers what sugar onto a precursor substance ?
Without this H gene (hh) a patient is said to have what blood type ?
L-fucose
Bombay
An individual has the following serologic reactions :
D+ , C + , E +, c+, e+, f-
What is the most probable genotype ?
R1R2
because f neg - can't have c, e together on same allele
What substances would be found in the saliva of a group B secretor who also has the Lele gene?
H, B , Lea, Leb
Once thawed , FFP must be transfused within ____ hours or relabeled as thawed plasma
24
For Autologous donation the donors Hgb should be
11.0 g/dl
for normal donation : 12.5 for women / 13.0 for men
A type O mom has a baby with a type B father . What is the possible genotypes of the baby?
BO or OO ( know genotype vs. Phenotype )
** if asked possible phenotypes : B and O
Rh antibodies are primarily of which immunoglobulin class ?
IgG
In what way do the Lewis antigens change during pregnancy ?
Lea and Leb both decrease .. this is why you can sometimes see increase in antibodies to these antigens
Quality control for nonadditive RBCs requires a maximum hematocrit level of :
80%
A donor had a positive anti-HAV IgM test 3 months ago. Are they eligible to donate blood ? If not how long are they deferred?
Eligible , no deferral - Hep A is not transmitted by blood transfusion
If that is Hep B or Hep C - permanently deferred ** some discussion if was Hep C before age 11
Se
What Rh antigen is found in 85% of the caucasian population and is always clinically significant?
D
A patient with an M. pneumoniae infection will most likely develop a cold autoantibody with specificity to which antigen?
I
Cryoprecipitate that is has been pooled in an open system must be transfused within ____ hours
4 hours
How long is a patient deferred from donation after having a blood transfusion ?
12 months
A patient who is type A2 would see which results with Anti-A reagent ( mixed with Pt red cells ) , what reaction would you see with mixed with Anti-A1 lectin? What is the Anti-A1 lectin called?
+ with Anti-A
0 with Anti-A1
A1 lectin = Dolichos biflorus
If a patient is R1R1 and is transfused with RBCs that are Ror, which antibody is most likely to be produced?
anti-c
This phenotype associated with the Kell blood group system is also associated with an X-linked chronic granulomatous disease
McLeod
irradiation of Red cells is done to destroy T lymphocytes in donor unit and prevent graft vs host disease. The recommended dose of gamma radiation is _____ Gy to the central portion of the unit and no less than ____ Gy to any part of the unit
25 and 15
How much time must you wait between allogeneic whole blood donations ?
56 days
Put these in order from greatest amount of H substance to least amount of H substance
A2B, A2, O, A1B, A1, B
O >A2>B>A2B>A1>A1B
patient 1 had the following results :
Anti-A = 4+
Anti-B = 0
Anti-D 1+
patient 2 had the following results :
Anti-A= 4+
Anti-B = 0
Anti-D = 4+
which patient would you believe to be :
Dce/dCe and which would be DCd/dce?
Why?
patient #1= Dce/dCe
Patient #2= DCe/dce
patient 1 has weaker expression of D antigen due to D and C being in trans position where patient 2 has stronger D expression due to D and C being in cis position.
This blood group system antibodies are often a common cause of delayed Hemolytic transfusion reactions
Patient's who are IgA deficient may develop anti-IgA which may stimulate an allergic reaction when exposed to a unit containing normal IgA .. these patients would benefit from ________ of the red cell unit before transfusion
Washing
immunization for rubella would result in a temporary deferral of how long?
4 weeks