Techniques
A technique that may miss weak antibodies by reducing reaction strength.
What is a prewarm?
Reacts with antigens of high frequency in the population.
What is a high-titer, low avidity antibody?
An antibody that reacts at IS phase but disappears following incubation and AHG phases?
What is anti-Lea?
Indicates the class of antibody based on IS, 37 C and AHG.
What is the phase of reaction?
Check transfusion and pregnancy history
What is the first step in a positive screen?
Red cells used in an autoadsorption to remove antibody from the serum.
What are patient red cells?
Antibodies that are not ABO antibodies and require previous transfusion and/or pregnancy to occur.
What are atypical antibodies?
Antibodies that are enhanced with enzymes.
What are Rh system antibodies?
IgM
What antibody class is positive at IS phase of an IAT?
Reagents used when the DAT is positive with polyspecific AHG?
What are monospecific anti-IgG and anti-C3 reagents?
A technique that disassociates IgG antibodies from red cells for further identification.
What is an elution?
Enzymes that are not used in routine screening as they may destroy some antigens causing missed antibody reactions.
What are proteolytic enzymes?
An antibody that is not produced against a low-incidence antigen.
What is anti-Vel?
IgG
What antibody class is positive at the incubation and AHG phases of the IAT?
On ruling out and matching the pattern, if only one cell is identified.
What is to test two more cells?
When a patients serum and Lewis substance reaction is negative (0) and the patients serum and saline control is positive (1+).
What is a neutralized antibody?
DTT (dithiothreitol)
What is the reagent useful for identifying ALL Kell blood group system antibodies?
The typical specificity of cold autoantibodies.
What is anti-I?
Extend incubation time, increase serum-to-cell ratio, use potentiators like PEG.
What can be done to increase sensitivity of an antibody screen in a compatibility test?
If a patient has a confirmed antibody but has phenotyped with the same antigen on their red blood cells.
Investigate recent transfusion history
The release of uncrossmatched, group O, D negative, RBC units without a patient sample.
What is an emergency release?
A medication likely to cause the production of autoantibodies
What is Methyldropa?
THIS QUESTOINS IS NOT IN A CATEGORY!!!
3%
How much does 1 unit of blood increase the hematocrit?
No prior or existing clinically significant antibodies are present.
When IS cross match is acceptable?
Positive DAT, False positive Fya, crossmatch incompatible at AHG.
What is autoimmune hemolytic anemia?