ABO Discrepency
see the following ABO results
Anti-A Anti-B A1 cells B cells
4+ 1+ 4+ 0
What is the patients most likely blood type and what is the discrepancy ?
IgG coated cells ( check cells ) must be added to negative reactions with AHG. This is to detect what ?
that AHG was added properly and that the cells were adequately washed.
What is the shelf life of whole blood collected in
CPDA-1?
35 days
Which of the following would be acceptable for blood donation?
a. 62 year old female with BP of 210/180
b. 18 year old female weighs 100lb
c. 40 year old male with pulse of 115
d. 82 year old male with hgb. of 13.5 g/dl
D
a is outside limit of BP systolic 90-180 and diastolic 50-100
b is below weight of 110lb
c is outside pulse limit of 50-100
The red cells of a nonsecretor will most likely type as
a. Le ( a-b-)
b. Le ( a+b+)
c. Le ( a+b-)
d. Le ( a-b+)
nonsecretors se/se cannot convert to Leb.. therefore can only be Le (a-b-) or Le ( a+b-).. most likely is Le (a+b-) .. so C is correct
if they were also le/le they could be Le (a- b-) but unlikely
See the following patient ABO testing
Patient cells Patient serum
Anti-A 4+ A1 cells 1+
Anti-B 0 B cells 4+
with additional testing below
IS RT
screening cell I 1+ 2+
screening cells II 1+ 2+
autocontrol 1+ 2+
What is the most likely cause of this discrepancy?
a. A2 with Anti-A1
b. cold alloantibody
c. cold autoantibody
d. acquired - A phenomenon
c.
C
A patient types as AB and appears to be Rh
positive on slide typing. What additional tests
should be performed for tube typing?
A. Rh negative control
B. Direct antiglobulin test (DAT)
C. Low-protein Rh antisera
D. No additional testing is needed
A
which of the following blood components will be best source of fibrinogen for someone with hypofinbrinogenemia
a. whole blood
b. FFP
c. platelets
d. cryoprecipitate
D
What are the requirements for hemoglobin for female allogenic donation, male allogenic donation and female/male autologous donations?
12.5 g/dl / 13.0 g/dl/ 11.0 g/dl
What antibodies could an R1R1 make if exposed to
R2R2 blood?
A. Anti-e and anti-C
B. Anti-E and anti-c
C. Anti-E and anti-C
D. Anti-e and anti-c
B. patient doesn't have E or c antigen and then have been exposed to the E and c antigen
Give a reason for the possible ABO discrepancy seen below and a way to resolve
Anti-A Anti-B A1 cells B Cells
4+ 0 1+ 4+
Dolichos biflorus 4+
Auto control Neg
Cold Alloantibody reacting with antibody on reagent A1 cells
*** If the result of Dolichos biflorus is Neg - this is what you would see with A2 with anti-A1
all of the following should be done immediately after suspected transfusion reaction called Except:
a. DAT on post transfusion sample
b. clerical check for clerical errors
c. visual exam of pre and post sample for hemolysis
d. gram stain on patients plasma
D
** first thing is clerical check
gram stain only if sepsis suspected .
What blood component should be used to help prevent HLA alloimmunization of the recipient?
Leuko reduced Red blood cells
Hep B immune globulin administration is how long of a deferral for blood donation?
12 months
A stem cell transplant patient was retyped when
she was transferred from another hospital. What is
the most likely cause of the following results?
Patient cells: Anti-A - neg Anti-B - 4+
Patient serum: A1 cells,- neg B cells - neg
A. Viral infection
B. Alloantibodies
C. Immunodeficiency
D. Autoimmune hemolytic anemia
C
Transplant patients take immunosuppressive meds to decrease graft vs. host issues. This will cause decrease in antibody expression and may not pick up back type expected ( with A1 cells in this situation)
The following results are obtained on routine ABO /Rh testing
cell testing Serum testing
Anti-A 0 A1 cells 4+
Anti-B 4+ B cells 2+
Anti-D 0
autocontrol - neg
select the course of action to resolve the problem:
a. enzyme treat the patient's red cells and repeat the forward blood typing
b. test the patient's serum with A2 cells and the patient's red cells with Anti-A1 lectin
c. repeat the ABO antigen grouping using 3x washed saline - suspended cells
d. perform antibody screening procedure at immediate spin and AHG using group O cells
D.
Unexpected reactivity with reverse cells should include a test with antibody screening cells at immediate spin to determine if alloantibodies are present . the discrepancy can be resolved by performing ABO testing using group B cells that lack the corresponding antigen for the identified antibody
A patient tests positive for weak D but also
appears to have anti-D in his serum. What may be
the problem?
D mosaic
a. remain the same
b. reduced by 4 days
c. reduced by 14 days
d. increased by 2 days
b.
when irradiated unit expires 28 days from irradiation or original expiration date whichever comes first
in order to donate platelet pheresis your platelet count must be at least ?
150,000/ul
which blood group system is mostly associated with delayed transfusion reactions
Kidd ( Jka or Jkb)
An ABO discrepancy that you may come across is an A2 with anti-A1 patient. What type of RBC can be transfused to these patients?
A or O
Anti-A1 typically is only reactive at RT and not clinically significant
a patient has a probable anti-Fya. However anti-c , and anti-K cannot be excluded. which of the following cells would be the most useful cell to exclude both anti-c and anti-K efficiently ?
a. Fya - Fyb +, C+, c+, K+k+
b. Fya - Fyb+, C- , c+, K+ k-
c. Fya - Fyb+, C+ c+, K+k-
d. Fya + Fyb- , C- c+, K+ k-
b
Negative for Fya and homozygous for c and K
Which of the following is responsible for causing transfusion associated graft vs host disease ?
a. platelets
b. granulocytes
c. monocytes
d. lymphocyte
D. lymphocytes
can be prevented by irradiation
which of the following defferal period is required for double red cell unit donation?
a. 1 year
b. 16 weeks
c. 8 weeks
d. 4 weeks
16 weeks
Which one of the Lewis blood group system phenotypes usually produces anti-Lea? ?
Le ( a-b-) almost exclusively are the makers of anti-Lea .. almost never Le (a-b+)