Blood bank
What is the most important test in blood bank?
What is ABO blood typing
Name in order the 3 most immunogenic blood group systems
What is
1) ABO
2)Rh
3)Kell
Which blood group system possesses the Jsb and Kpa antigens?
What is Kell
What are the 3 outcomes for a person who wants to donate?
What is accepted, temporary deferral, permanent deferral
How many mLs of anticoagulant is found in a 450 mL bag?
63
What test detects serologic incompatibility between donor RBCs and recipient serum?
What is crossmatch
What is anti-IgG and anti-C3d
According to Landsteiner's rule, if a patient has no ABO antibodies after serum testing, what ABO antigens are present on the patient's red cells?
What is both A and B
What ABO phenotype would agglutinate in the presence of anti-A,B produced by group O individuals?
What is A and B
What are the two things enzymes do to antigens?
What is enhances activity or destroy antigens
Which blood group system's antigen are associated with glycophorin A and glycophorin B
A male donor has a reactive nucleic acid test for hepatitis C. What kind of deferral is assigned to this donor?
What is permanent deferral
RBC's need to be transported at what temperature and on what?
What is 1-10 C and transported on ice
When a patient with a clinically significant antibody requires a blood transfusion, what type of RBCs must be chosen for them?
a. Irridated RBCs
b. ABO compatible
c. Antigen negative for antibody found
d. No specific requirement
What is C (antigen negative for antibody found)
What is the test procedure that combines patient's serum with commercial A1 and B reagent red cells?
What is ABO reverse (back) grouping
What term describes using known sources of reagent antisera (known antibodies) to detect ABO antigens on a patient's red cells?
What is forward (front) grouping
What two tests have to correlate to have a valid ABO type?
What is forward typing (front) and reverse typing (back)
What is the immunoglobulin class of most Rh system antibodies?
What is IgG
What reagent destroys the Kell system antigens?
What is DTT (dithiothreitol)
Following donation, how long does it take to replace the fluid lost from giving whole blood?
What is 72 hours
What blood product is tested for bacterial contamination following storage?
What is platelets
A recipient with group A phenotype requires a transfusion of 2 units of frozen plasma. What types are appropriate to for transfusion?
What is AB and A
What are Coombes check cells coated with?
What is IgG
How is the Rh genotype CDE/cDE writtne in Wiener notation?
What is RzR2
An excess of antibodies in a sample can cause what effect?
a. prozone
b. postzone
c. zone of equivalence
d. lattice formation
What is A
Anti-M usually reacts at room temperature and is typically IgM. Based on this information, is this antibody clinically significant? Why or why not?
What is No
-React at room temp
What is a null phenotype? Give 1 example of a null phenotype
What is don't present with antigen example is Rhnull
What is the minimum weight of allogenic blood donation?
What is 110
What measures are taken to prevent cytomegalovirus infection from blood units?
What is leukocyte reduction
What test are included in compatibility testing?
What is blood typing of recipient, antibody screening on recipient and crossmatch
To distinguish between an A1 and A2 blood type, which reagent is used?
What is Dolichos biflorus lectin
Testing for the weak D expression is performed by:
Performing the indirect antiglobulin test with anti-D
A patient's red cell are agglutinated by anti-B, but not by anti-A. What is this patient's ABO phenotype?
What is Group B
Name the main two antigens in the Kell blood system
What is K/k
What disease is commonly associated with the McLeod phenotype?
What is chronic granulomatous disease
Before donation, the intended venipuncture site must be cleaned with
What is PVP-iodine complex
What temperature does platelets need to be stored at?
What is room temperature
What ABO phenotype would be compatible if the patient required a transfusion of RBCs?
What is only group O
After adding antigen and antibody to a test tube, one large agglutinate was observed. How should this reaction be graded?
What is 4+
What patient phenotype as D+C+E-c-e+. Predict the most likely genotype
What is R1R1 (could also have r, but that is rare a group)
Why is it sometimes necessary to distinguish A1 and A2 blood types?
What is to resolve a discrepancy between the forward and reverse typing
Most "naturally occurring" ABO system antibodies fall into which immunoglobulin?
What is IgM
Name two antigens of the Lutheran blood system
What is Lua and Lub
A donor tested D-negative using commercial anti-D reagent. The weak D test was positive. How should the RBC unit be labeled?
What is D-positive
To prevent graft-versus-host disease, blood components prepared for a fetus that needs an intrauterine transfusion should be:
What is irradiated
What ABO phenotype would be compatible if the patient required a transfusion of fresh frozen plasma?
What is AB, O, A or B
True or False
Both direct and indirect antiglobulin test detects IgG and complement-coated red cells.
What is true
The weak D test detects:
What is a weak D antigen