Blood Cultures
Site and Arm Preparation
100

Why might a doctor order a set of blood cultures? 

BC aid in identifying specific organism causing the infection 

Patients who have fevers of unknown origins

100

What are the first 4 steps in site preparation for Blood Culture Collection

Prepare and assemble supplies

identify patient, Explain test

Wash or sanitize hands, put on gloves

After applying tourniquet locate the vein

200

How might Blood Cultures differ from a regular blood draw?

As a phlebotomist you must explain the procedure in greater detail to the patient 

Puncture site must be decontaminated so that it is sterile

200

What type of disinfectant are you using to clean the site?

ChloraPrep 

2% Chlorhexidine Gluconate and 70% Isopropyl Alcohol 

300

How do Culture bottles differ from regular blood tubes?

BC bottles contain culture media that enables bacteria to enable bacteria to grow under lab conditions 

300

How long do you cleanse the site?

60 Seconds 

400

What are some interfering factors with Blood Cultures?

Blood Culture specimens must be collected first 

Do not scrape the needle accross the skin

fill aerobic blood culture vial first

culture bottles containing resin beads

500

Can you overfill a Blood Culture Bottle?

YES! Overfilling could cause a false positive  

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