DNA and genetics
This molecule carries genetic instructions for the development, functioning, and reproduction of all known organisms.
What is DNA?
This tool is used to introduce foreign DNA into a host cell, such as a bacterium, often in the form of a circular plasmid.
What is a vector?
This microorganism is commonly used in biotechnology for cloning, gene expression, and production of recombinant proteins.
What is E. Coli?
This hormone is primarily responsible for the growth and maturation of ovarian follicles.
What is FSH?
These enzymes unwind the DNA helix during replication.
What are helicases
This process is used to make many copies of specific DNA segments in a laboratory
What is PCR
This type of library contains DNA sequences that are reverse-transcribed from mRNA, representing actively expressed genes.
What is a cDNA library?
This blue/white screening marker is used to identify colonies that have successfully incorporated foreign DNA.
What is lacZ (or β-galactosidase)?
This early-stage ovarian follicle is small and surrounded by a single layer of granulosa cells.
What is the primordial follicle?
This enzyme is responsible for synthesizing DNA from an RNA template.
What is reverse transcriptase
The complete set of DNA, including all of its genes, in an organism.
What is a genome?
This technology allows scientists to cut and edit genes with high precision
What is CRISPR?
This term refers to organisms that have been genetically modified to contain genes from other species.
What are transgenic organisms?
This more mature ovarian follicle contains a fluid-filled cavity and responds to FSH
What is an antral follicle?
This type of enzyme is responsible for "sealing" DNA fragments during cloning by forming phosphodiester bonds.
What is DNA ligase?
This type of enzyme cuts DNA at specific sequences, acting as molecular scissors in genetic engineering.
What are restriction enzymes?
In gel electrophoresis, DNA fragments are separated based on this physical property
What is size (or molecular weight?)
This bacterium, which naturally transfers part of its DNA into plants, is commonly used in genetic engineering for creating transgenic plants.
What is Agrobacterium tumefaciens?
This process involves stimulating the ovaries to produce multiple follicles in one cycle and can be achieved using FSH or eCG.
What is superovulation
This enzyme is responsible for adding nucleotides to the growing DNA strand during DNA replication.
What is DNA polymerase?
These sequences of DNA are non-coding, but may regulate gene expression and play structural roles.
What are introns?
In this type of mutagenesis, specific nucleotide changes are introduced into a gene to study its function.
What is site-directed mutagenesis?
This type of gene transfer in bacteria involves the uptake of free DNA from the environment.
What is transformation?
These cells are responsible for producing testosterone in response to LH.
What are Leydig cells?
This method is used to separate proteins based on their size.
What is SDS page?