Trivia
DNA Replication
PCR
Gene Regulation
Random
100
Who is this?
IU
100
What is one key difference between DNA and RNA?
Answers can vary:
Nucleobase Uracil vs Thymine
Deoxyribose/Ribose
100
What does PCR stand for?
Polymerase Chain Reaction
100
A diagram of the lac operon will be drawn on the board. Fill in the blanks!
10 points per each blank filled.
100
Guess the price! closest answer wins!
$850
200
What brand of alcohol is this?
Kirin
200
In which direction can dNTPS be added in DNA or RNA synthesis?
Always in the 5' -> 3' direction.
200
What are the 3 steps of PCR in order(include ideal temperatures within +/- 5 degrees)?
1. Denature - 95 degrees
2. Anneal - 60 degrees
3. Elongate - 72 degrees
200
What does lacZ transcribe for and what is its purpose?
lacZ codes for production of beta galactosidase which hydrolyze lactose in glucose and galactose
200
SIDE GAME: First two people to buzz in will play each other in CONNECT4 for a chance to win the points!
Points go to winner!
300
What is the capital of Indiana?
Indianapolis
300
What is the difference between a leading and lagging strands?
Leading(or continuous) strand is the strand that is synthesized towards the replication fork. Lagging(or discontinuous) strand is the strand that is synthesized in the direction away from the replication fork.
300
What can you find in a typical PCR test tube?
One double stranded DNA, Primers, DNA polymerase, and dNTPs
300
If a mutation were to occur leading to the lack of the lacY gene on the lac operon, how would this affect the bacteria?
As the lacY gene codes for permease to allow for more lactose to enter the cell, you can assume that the lack of this gene would lead to less energy produced.
half points for mentioning lacY codes for permease/half points for giving acceptable outcome
300
Riddle me this!
There’s a one-story house in which everything is yellow. Yellow walls, yellow doors, yellow furniture. What color are the stairs?
There are no stairs, it's a one story house.
400
What can you break, even if you never pick it up or touch it?
A promise
400
Explain the difference between eukaryotic and prokaryotic replication bubbles.
The replication bubble forms at a specific sequence of bases called the origin of replication. Bacterial chromosomes have only one origin of replication, and they form a single replication bubble. Eukaryotes have multiple origins of replication along each chromosome, forming multiple replication bubbles.
400
Why is DNA Polymerase I not used in PCR?
There are no RNA Primers to remove.
400
Describe the role of CAP and cAMP?
CAP and cAMP work together to enhance RNA polymerase binding
400
Which one have I dated:
Bottom Left
500
Schoenberg & Webern were noted for pioneering use of this type of musical scale.
12-tone or 12-note chromatic scale.
500
Name and explain all the enzymes used in DNA Replication of either the leading or lagging strand.
Helicase - unzips strands
SSBP's - used to stabilize single stranded DNA
Topoisomerase - breaks and rejoins the DNA double helix to relieve twisting forces caused by the opening of the helix
Primase - catalyzes synthesis of RNA primer
DNA Poly. III - extends leading strand/Okazaki Fragments
DNA Poly. I - replaces RNA primers with DNA
DNA Ligase - Catalyzes joining of Okazaki fragments into continuous strands
500
In your PCR lab, your fellow researchers propose three different methods for 20 cycles of PCR. The first proposes to double the temperature. The second proposes to halve the cycle. The last proposes to use 4x the materials but only 1/4 of the cycles. Which process will have amplified the most DNA?
The one who halved the number of cycles
500
What are the difference between Eukaryotic and Prokaryotic Gene Regulation?
Eukaryotic transcription regulation
- activator binds to enhancer
- repressor binds to silencer
- include presence of mediators that bind to activators, and general transcription factors
- RNA pol binds to core promoter
- information organized in singular genes
Bacterial transcription regulation
- activator binds to unique binding site (such as CAP binding site) upstream of core promoter
- repressor bind to operator
- RNA pol binds to core promoter
- information organized in multiple subsequent genes called operons
500
DAILY DOUBLE: Truth or Dare!
Group Decided Truth or Dare!