Cell Cycle
What are the 3 phases of interphase ?
G1- Grow S- Syntesis G2- Grow again
What are the four stages of mitosis (In order)
Prophase Metaphase Anaphase Telophase
What technique is used to modify DNA to make GMOs?
Recombinant DNA
What is it called when you make an identical copy of the cell.
cloning
During which stage do sister chromatids line up in the middle of the cell?
Metaphase
Type of treatment of cancer that uses viral vector to insert working copy of a gene.
gene therapy
What does GMO stand for?
genetically modified organism
What happens in somatic cell nuclear transfer?
somatic cell nucleus is inserted into an enucleated egg cell
What is the job of the M check point?
To make sure the chromosomes are lined up correctly.
What type of genes cause an increase in cell division?
proto-oncogenes
What is the purpose of PCR?
to amplify a segment of DNA
What is CRISPR used for?
modifying DNA
What is the point of the G2 checkpoint?
To make sure DNA replication occurred correctly.
A mutation is a p53 gene leads to increased cell growth and tumor formation. What type of gene is p53 and what does it control?
tumor suppressor, suppresses growth by triggering apoptosis
What technique separates DNA based on size?
gel electrophoresis
insulin producing bacteria, modified crops
What is the result of Mitosis (make sure to include the type -ploidy)
A production of 2 new daughter cells that are identical to the parents cells
A mutation in a specific gene (______________) leads to an increase in mitosis. The gene then is referred to as a _______________.
proto-oncogene, oncogene
What is the Sanger method?
sequencing technique that uses dideoxy nucleotides
What is the purpose of a restriction enzyme? and
Name 1 way it is used.
cut DNA at a specific sequence
insert gene into plasmid, gel electrophoresis