DNA Technology
Production of identical copies of an organism, cell or DNA through asexual means
What is cloning?
The use of natural biological systems to create a product or achieve some other end.
What is biotechnology?
DNA knowledge enabled gene manipulation
What is DNA technology?
This is the production of identical copies of an organism, cell or DNA through asexual means.
What is cloning?
The use of natural biological systems to create a product or achieve some other end.
What is biotechnology?
A technique used to create billions of copies of a segment of DNA in a test tube in hours.
What is PCR?
Organisms that have had a foreign gene inserted into their genome.
What are transgenic organisms?
The process of modifying genomes to improve an organism's characteristics, make biotechnology products, or treat cancer and genetic disorders.
What is genetic engineering?
This type of protein is used to cleave vector DNA. Hundreds occur naturally in bacteria and are a part of their primitive immune system against viruses.
What are restriction enzymes?
This procedure determines the order of nucleotides in a segment of DNA.
What is DNA sequencing?
This enzyme cleaves vector DNA.
What is a restriction enzyme?
Biotechnology products produced by what transgenic organism include insulin, human growth hormone, tPA (tissue plasminogen activator), and hepatitis B vaccine.
What is transgenic bacteria?
RNA sequences that are complementary to the mRNA transcribed by a certain gene. These RNA pieces are used to "silence" expression of specific alleles. They do this by binding the target RNA and producing a double-stranded RNA molecule which is then broken down by enzymes (double-stranded RNA cannot be translated).
What is RNA interference (RNAi)?
What are antibiotics?
What is complementary DNA or cDNA?
This enzyme will seal foreign DNA into the opening of the DNA vector created by the restriction enzyme.
What is DNA ligase?
This bacteria is a very commonly used bacteria when it comes to producing biotechnology products.
What is E. coli?
This type of DNA technology can be used to identify the source of bacterial or viral pathogens.
What is PCR?
The biggest discovery of the human genome project.
What is the discovery of single nucleotide polymorphisms (SNPs)?
Applications from DNA technology can be used for what four areas as I described on my slides?
What are therapeutic applications, genome projects, scientific applications, and agricultural applications?
This type of gene editing was first discovered in prokaryotes, acting as immune defense against invading viruses.
What is CRISPR?
What are genetically modified organisms or GMOs?
This is when DNA comes from two or more sources. To make this we need a vector, often a plasmid, restriction enzymes to cut the vector, and a gene of interest to insert into the vector.
What is recombinant DNA (rDNA)?
The percent of the human genome DNA that does not directly code for amino acid sequences.
What is 98%?
This organism can often secrete a gene product continuously.
What is yeast?