Key Discoveries
What type of enzyme can precisely cut DNA?
Cas9
What does CRISPR-Cas9 cut?
Target DNA / DNA that matches the crRNA sequence
Which enzyme did Doudna and her team use in experiments to cut DNA at precise locations?
Cas9
Which CRISPR system is found in bacteria like E.coli?
Type 1 CRISPR
What does CRISPR stand for?
Clustered Regularly Interspaced Short Palindromic Repeats
What experimental marker did scientists use to test the effectiveness of CRISPR-Cas9 in gene editing?
Green Fluorescent Protein(GFP)
What breakthrough simplified the CRISPR-CAS9 system?
Single guided RNA
What was CRISPR's function in Bacteria?
An adaptive immunity system to protect against viruses.
What element was injected with the DNA to view the gel bands under X-Ray film?
Radioactive Phosphorus
Who played a big role in the invention of sgRNA with Dr. Doudna?
Emmanuelle Charpentier
What is sgRNA made of?
TracrRNA and crRNA
What laboratory technique was used to analyze DNA fragments after CRISPR cuts?
Gel Electrophoresis
Which bacteria was used to understand the CRISPR-CAS9 system?
Streptococcus Pyogenes
Which end of a crRNA strand does a DNA repair template need to be attached to?
For effective CRISPR use, the DNA repair template needs to be attached to the 3' end of crRNA
How many different sgRNA's did scientists engineer when testing the jellyfish DNA?
5