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ALA (delta aminolevulic acid) porphyrin test is used to determine if?
The organism has the ability to produce heme
Parainfluenzae will be positive
How do you differentiate Haemophilus influenzae from parainfluenzae?
Influenzae requires X and V factors (ALA negative)
Para only requires V (ALA positive)
Who is apart of the HACEK group?
Haemophilus
Aggregatibacter
Cardiobacterium
Eikenella
Kingella
What organism is responsible for purulent conjunctivitis "pink eye"?
Haemophulis influenzae biogroup aegyptis
What are the pathogenic Haemophilus?
H. influenzae and H. ducreyi
Boxers are most likely to get this organism, why?
Eikenella
Because they bite each other and have open wounds that get spit on
What are two requirements that may be necessary for Haemophilus growth?
Factor X (Hematin or heme)
Factor V (NAD (nicotine adenine dinucleotide))
What diseases do Legionella pneumophilia cause?
Legionellosis (Legionnaries' disease and Pontiac fever)
This organism grows as granules or aggregates in broth cultures and rare cause of endocarditis?
Aggregatibacter
This organism has thing GNB with tapered ends and a gliding motility on SBA that can be a cause of periodontitis in diabetics and alcoholics and present in normal dog flora?
Capnocytophaga
Haemophilus influenzae requires what factors?
X and V factors, will be ALA negative
This organism is a short coccobacilli and may pit agar surface and a rare cause of endocarditis?
Kingella
Why does Haemophilus not grow on SBA? When is the only time that Haemophilus grows on SBA?
It has NADase
Satellitism around S. aureus
This bacteria pits the surface of the agar is a corroding bacterium and bleach like odor?
Eikenella
What agars does Haemophilus grow on?
Chocolate and Horse blood with bacitracin
NAD (nicotine adenine dinucleotide (NAD) is derived from?
S. aureus, potato, and yeast
This STI has a chancroid and on gram stains of direct genital is school of fish?
Haemophilus ducreyi
This organism gram stains poorly? What can be done to fix the staining?
Legionella
Substitute Giemsa or basic fuchsin for counterstain (Safranin)
What is Hematin or heme (Factor X) derived from?
Hemolyzed red blood cells
What stage of Bordetella pertussis has the signature whooping cough?
Paroxysmal
What can Legionella be isolated on and from what speciment?
Buffered charcoal yeast extract (BCYE)
Urine and other body fluid
Who is the most at risk group for Haemophilus influenzae?
Nonimmunized infants
What does Bordetella pertussis look like on Bordet gengou media?
Shiny mercury like colonies after 7-10 days
Why is nontypeable Haemophilus influenzae becoming the majority of H. influenzae infections?
Hib immunization lead to decrease of typeable strain. Vaccine does not kill nontypeable strain
This organisms causes small alpha hemolytic colonies on SBA and may pit agar surface and a rare cause of endocarditis?
Cardiobacterium