Bioethics + 3 Rs
What are the 3 Rs?
-Reduction-Study only uses the minimum
-Refinement-Refine a procedure to reduce pain and distress
What do the abbreviations GLP and SOP stand for?
GLP-Good Laboratory Practices
SOP-Standard Operating Procedures
______ is a standard form developed by the institution. It describes and justifies the proposed study while providing information required for regulatory compliance.
Protocol
The trasnportation, care, and use of of animals should be in accordance with the ____ ____ _____.
Animal Welfare Act
What is the primary source of the standards and guidelines for laboratory animal care and use in the united states?
The Guide for the Care and Use of Laboratory Animals (The Guide)
What is the study of social and moral issues concerning work with live animals for research and the applications of that research?
Bioethics
What is the purpose of an SOP?
They are written step by step instructions of how a procedure should be done approved by management and used to perform a routine activity.
True or false: A protocol is usually assigned a color. This usually identifies all animals, cage cards, documents, and health records associated with the protocol.
False: Protocols are assigned an ID number based on the institutions numbering scheme
True or False: Surgical or other painful procedures should not be preformed on unanesthetized animals paralyzed by chemical agents.
True
Name 5 components of animal care and use that are described in The Guide
-Personnel qualifications and training
-Occupational health and Safety
-Animal environment, housing, and management
-Veterinary Care
-Animal Procurement and transportation
-Surgical and postsurgical care
-Alleviation of pain and distress
-Euthanasia
-Physical plant design and construction
Name 3 ways to maintain a balance between the use of animals in research and the benefits of said research
-Work is scientifically relevant
-Animals maintained in ways that provide physical and psychological well being
-Animals not subject to unnecessary pain and distress or pain and distress is minimized
-Humane euthanasia in order to end suffering that cannot be relieved
What is the purpose of a GLP?
To validate and document the processes by which safety tests are conducted in such a way that the process can be reconstructed if needed.
Based on The Guide an IACUC Board must consist of?
A. Veterinarian with training or experience in lab animals medicine, Scientist using animals, non scientist, and a public member
B. Scientist experienced in animal research, Veterinarian with training or experience and has program authority, Nonscientist, Nonaffiliated member
C. Chair Person, Veterinarian with training or experience in lab animals medicine, Nonaffiliated member
A
True or False: Investigators and other personnel shall be appropriately qualified and experienced for conducting procedures on living animals. If not they can receive in-service training on study.
False: Adequate arrangements should be made for their in-service training.
In 1966 the United States Congress enacted the first federal law for laboratory animal welfare. What was it called?
The Animal Welfare Act (AWA)
What is the purpose of the 3 Rs?
To promote the development and practice of more humane experimental techniques and to reduce the amount of distress inflicted upon laboratory animals.
GLPs are funded and reviewed by who?
FDA (Food and drug Administration)
Based on The Animal Welfare Regulations an IACUC Board must consist of?
A. Veterinarian with training or experience in lab animals medicine, Scientist using animals, non scientist, and a public member
B. Scientist experienced in animal research, Veterinarian with training or experience and has program authority, Nonscientist, Nonaffiliated member
C. Chair Person, Veterinarian with training or experience in lab animals medicine, Nonaffiliated member
C
What is the full name of The US Government Principles?
The US Government Principles for the utilization and Care of Vertebrate Animals Used in testing
What defines the roles of the different individuals in the animal facility?
The Animal Welfare Regulations (AWR)
The zoologist William Russell and the microbiologist Rex Burch published what in 1959?
The 3Rs Principle
Name 2 requirements an animal facility must comply with to meet GLP standards
-Adequately train personnel and maintain a current training file (includes training documentation) job description, and training certifications)
-Establish QA
-Write SOPs for all husbandry and scientific procedures
Based on The Public Health Service an IACUC Board must consist of?
A. Veterinarian with training or experience in lab animals medicine, Scientist using animals, non scientist, and a public member
B. Scientist experienced in animal research, Veterinarian with training or experience and has program authority, Nonscientist, Nonaffiliated member
C. Chair Person, Veterinarian with training or experience in lab animals medicine, Nonaffiliated member
B
For exceptions to the principles the decision lies with who?
Appropriate review board: IACUC
Which animal is not covered by the AWA and AWR?
A. Birds
B. Rats
C. Mice bred for research
D. All of the above
D