Requirements For Growth
What are the physical requirements for growth?
What is proper temperature, proper pH, and proper osmotic pressure
How many bacteria/ml in a students drink if a standard plate count procedure generated 53 colonies on the plate diluted 1:1000 and 0.1ml of the dilution was used as the inoculum?
What is 530,000?
What are the two types of growth/bacterial division?
What is binary fission and exponential growth?
What are the direct methods of growth?
What is direct microscopic count, plate count, and membrane filtration?
What is Quorum sensing?
What is cell to cell communication?
What are the chemical requirements for growth?
What is carbon, nitrogen, phosphorus, and sometimes oxygen?
How many bacteria/ml in a students drink if a standard plate count procedure generated 25 colonies on the plate diluted 1:1000 and 1ml of the dilution was used as the inoculum?
N/A because the colony size is less than 30
2-4-8-16-32-64-128
What is the length of a generation time?
What is 1 hour?
What are the indirect measurements for growth?
What is a spectrophotometer?
(To see turbidity)
What are the steps for a pour culture?
What is...
1. dilute the sample
2. mix with melted agar
3. pour into Petri dish
What toxic by-products kill anaerobes? And what enzymes do they lack?
What is superoxide radical and peroxide anion?
What is superoxide dismutase and catalase?
How many bacteria/ml in a students drink if a standard plate count procedure generated 34 colonies on the plate diluted 1:10 and 1ml of the dilution was used as the inoculum?
What is 340?
How long does it take E. coli and Mycobacterium to double?
What is 20 minutes and 12 hours?
Plate count disadvantages and advantages?
What is overnight incubation, and only viable (live) cells counted?
What is...
1. dilute the sample
2. inoculate small volume on surface of agar plate
3. Spread
What is a buffer?
What is maintains proper pH in culture media?
A sample of milk is tested for its bacterial content in a plate count assay. A 1 ml sample of the milk is diluted in a 1:10 serial dilutions. 1ml of the third dilution tube is plated in a pour plate. After incubation, the plate has 54 colonies, indicating that the original milk sample contained ________ cells per milliliter.
What is 54,000?
An experiment began with 4 bacterial cells in log phase and ended with 128 cells. How many times the cells divided?
What is 5?
What are the disadvantages and advantages to the Petrol-Hausser Counter? And what is it best for?
What is motile bacteria, dead bacteria, and concentrated sample?
What is quick results?
What is non-motil bacteria?
What is the equation for bacteria/mL?
What is bacteria/mL=(# of colonies)(inverse of the dilution factor)?
What are the examples for faculatative anaerobes, aerotolerant, and microaerophilic?
What is E. coli, Lactobacillus, and Streptococcus?
A sample of ocean water is tested for its bacterial content in a plate count assay. A one-milliliter sample of the water is diluted in a 1:10 dilution series. 0.1 milliliter of the second dilution tube is plated in a pour plate. After incubation, the plate has 191 colonies, indicating that the original water sample contained ________.
What is 191,000?
Three bacterial cells were inoculated into a culture medium with a generation times of 60 minutes. Assuming no lag phase takes place, how many cells are there after 5 hours?
What is 96?
Absorbance=concentration, the higher the concentration means...?
What is the more turbid (more dense) it is and the higher the absorbance value is?
What are fastidious organisms?
What is complex growth requirements and difficult to grow on artificial media?