Bacterial Growth
Bacterial Growth II
culture media
culture media II
Salinity
100
Time to double the population
What is Generation time
100
10 minute generation time
What is Bacillus Subtilis
100
Complex polysaccharide, used as solidifying agent for culture media in Petri dishes, slants, and deeps
What is Agar
100
Selective and differential, Ferment mannitol to acid, color change
What is Mannitol Salt Agar MSA
100
Hypertonic environments, or an increase in salt or sugar, cause plasmolysis
What is Osmotic Pressure
200
20 minute generation time
What is E. Coli
200
24 hour generation time
What is Mycobacterium Tuberculosis
200
Exact chemical composition is known
What is Chemically Defined media
200
Selective and Differential, Basic dyes, inhibits growth of gram positive, lactose fermenters produce dark purple colony
What is Eosine methylene blue agar EMB
200
Require high Osmotic pressure and Salt
What is Extreme or Obligate Halophiles
300
No growth, cells synthesizing enzymes needed to metabolism of new nutrients in environment
What is Lag phase
300
Exponential growth - growth > death More susceptible to antimicrobial drugs that target cell wall synthesis or metabolism
What is Log phase
300
Suppress unwanted microbes and encourage desired microbes
What is Selective Media
300
Beta hemolytic of blood on Blood Agar (complete) (only one to Beta) (yellowing)
What is Streptococcus pyogenes
300
-halophiles
What is Salt loving
400
Death = Growth
What is Stationary phase
400
Nutrients depleted and wastes (many times are toxic) increase metabolic rate declines
What is Stationary phase
400
Make it easy to distinguish colonies of different microbes, bacteria of interest has specific and identifiable reaction
What is Differential Media
400
Alpha (partial) hemolytic of blood on Blood Agar, growth with some color change
What is Streptococcus pneumonia
400
Tolerate high Osmotic pressure, don't need salt but okay with it
What is Facultative Halophiles
500
Endoscopes may be produced, Death > Growth
What is Death or Decline phase
500
Best time for gram staining
What is Log phase
500
Plates in sealed jar with gas pack or candle that removes oxygen producing anaerobic environment
What is Anaerobic Jar
500
Gamma (no) hemolysis of blood on Blood Agar Plate, growth no color change
What is Enterococcus Faecalis
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