Optical Techniques
A test species used that corrects for colour due to reagent colour used in the method
What is a reagent blank?
what is oxidized?
phase (solid or liquid) through which the mobile phase (our sample) flows through
the strength of the bond between the antigen and the antibody
What is affinity?
monosaccharides that make up maltose
glucose + glucose
Most common choice of light separation device on specs due to it's narrow band pass and linear dispersion of light
What is diffraction gratings?
half-cell that is used as a fixed reference of known voltage
what is a reference electrode?
Techniques used to separate complex mixtures based on different physical interactions between the individual compounds and the stationary phase of the system
unit of osmolality
mOsm/kg of water
type of diabetes where beta cells produces little to no insulin
lamp source of choice for viewing 400-700nm in a spec
What is quartz-halogen or tungsten-iodide
an indicator electrode specific for ions
ion-specific electrode (ISE)
a classic liquid chromatography, but a high-pressure pump drives the mobile phase instead of gravity.
what is high performance liquid chromatography (HPLC)?
In freezing point osmometry, the more ________ the freezing point is, the higher the concentration of analyte there is.
what is depression?
hormones that increase blood glucose levels
What are glucagon, growth hormone, epinephrine, glucocorticoids, and ACTH
Type of relationship between light reflected and concentration of the sample for reflectance photometry
What is NON-LINEAR
the PO2 electrode, aka the Clark electrode
an analytical instrument that ionizes a target molecule and then separates and measures the mass of its fragments.
what is a mass spectometer?
the compounds that make up osmolality
Na, glucose, urea
2(Na) (mmol/L) + glucose (mmol/L) + urea (mmol/L) = mOsm/kg
reference range for normal fasting (a.c) glucose
What is 4.1 - 5.5 mmol/L?
lamp of choice for visualization of 100-300nm
What is Hydrogen Deuterium?
the separation of proteins according to their isoelectric point (the pH value of when they are neutral charged) Hint: it was part of our independent reading.
iso-electric focusing (we also had to know dimensional electrophoresis)
an ionization technique used in mass spec where energized electrons interact with the target molecule and produces ions
What is electron impact
analyte in the test sample is measured by its ability to compete with labelled antigen for a limited number of antibody binding sites
reference range for diabetic P.C
what is 5 - 10mmol/L?