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Antibody screens are used for? (6 answers)
Patients requiring transfusions, pregnant women, patients who have had transfusion reactions, Blood/plasma donors, patients with autoimmune hemolytic anemias, and patients with graft vs host disease/complications of transplantation.
Proteolytic enzymes destroy which antibodies?
Duffy, and MNS
What antibody reacts with donor and self RBCs, and all reagents regardless of antigens present?
Autoantibodies
One unit of RBCs should increase the HCT and HBG by how much?
Increase the HCT by 3% and the HGB by 1 mg/dL.
Who can recieve D-negative units?
D-negative recipents or recipents who have developed anti-D
Rh, Kell, Kidd, Duffy, Sus, Lu b. React in AHG/IAT at 37C.
Proteolytic enzymes enhance which antibodies?
Rh, Kidd, and Lewis
What insignificant IgM antibody will react in the IS crossmatch phase, and sometimes at 37C?
Cold-ALLOantibodies
In order to use electronic crossmatching..?
The recipent must not have an antibody today or ever
D-positive units can be given to who?
D-positive recipients or D-negative recipents NOT of child-bearing age
If the autocontrol is positive after a positive antibody screen, what test do we run next to determine if an alloantibody or an autoantibody present?
DAT
What would you suspect if most panel cells are postive in AHG and autocontrol is negative?
An alloantibody to a high frequency antigen
What antibody will react in IS crossmatch, autocontrol, and DAT?
Cold-AUTOantibody
The IS crossmatch is preformed when...?
The recipient has no evidence of an antibody today or ever
Antigen-negative units are recomended for recipients with which antibodies?
ABO, Rh, Kell, Duffy, Kidd (R. Kelly Does Kids...)
If the autocontrol is negative what type of antibody is present?
ALLOantibody
What would you suspect if the antibody screen is negative and the crossmatch is positive?
An antibody to a low incident antigen
What technique is used to remove autoantibodies?
Adsorption
AHG phase is preformed when..?
Recipent has or has ever had a clinically significant antibody
What are the most common cold alloantibodies and cold autoantibodies?
Cold ALLO- Anti; P1,M,N,Le a, and Le b
Cold AUTO- Anti; I,H, and IH
If the DAT is positive what kind of antibody are we dealing with?
AUTOantibody
Can you phenotype a patient who has been transfused in the in the past 3 months?
No
What technique detaches IgG from the patients RBCs?
Elusion
A crossmatching segment should be kept for how long?
7 days
An autocontrol is made by using what?
The patients serum/plasma and RBCs