Chromatography
Separates mixtures into individual components.
What does Chromatography do?
Paper
What is the stationary phase of Paper chromatography?
TLC
What is Thin Layer Chromatography?
Size and shape of the molecules
What is "how molecules are separated in gel(sieve) chromatography?"
Matrix
What is "agarose"?
The fluid portion of Chromatography
What is the mobile phase?
Solvent
What is the mobile phase of paper Chromatography?
Stationary phase
What is thin glass plate coated with matrix.
Filling the chromatography column with matrix.
What is "packing" the column?
Polarity, shape, and molecular weight
What is "how gel electrophoresis separates molecules"?
Term for the non moving portion Chromatography
What is stationary phase?
The solubility of a particular solvent with components of a mixture.
What is the determining factor in how far the components of the mixture separate and travel up the paper stationary phase?
Differences in polarity
What is " how TLC separates substances"?
Order the largest molecules come out of column.
What is "first"?
The one (900bp or 500bp) that moves in the gel the fastest.
What is the "smallest molecular weight 500bp"?
Paper, Coated glass plates, bead slurries, agarose
What are stationary phases of various types of Chromatography?
Term that describes the chemical activity of the stationary phase.
The furthest point reached by the solvent.
What is the "solvent front"?
A rod and sphere have the same weight and this one elutes off of the column first.
What is the "rod"?
Causes the movement of mobile phase
What is, "electric current carried through buffer mobile phase"?
Three industries that utilize Chromatography.
What are Pharmaceuticals, Environmental, Forensics, and Biochemistry?
Capillary action and diffusion
Rf
What is "Retention factor"? dye front/solvent front and is always 1 or less
Trouble separating out analytes that are similar in size, try this.
What is, "run a longer column"(change column length)?
How to get better resolution on 100bp fragment.
What is, "increase gel agarose % to 2, or 3%, slow gel down voltage, or make a longer gel"?