Introduction
Spectrophotometry
Proteins
Albumin &
Total Protein
Urine &
CSF Protein
100

This many microliters are in 1 liter.

What is 1x10^6 or 1,000,000?

100

Energy of a lightwave has this type of relationship with its wavelength.

What is inverse?

100

Serine is classified as this type of amino acid.

What is polar?

100

These are the reference ranges for serum albumin and total protein.

What is 3.5-5.0 g/dL and 6.0-8.0 g/dL?
100

This type of proteinuria can come from cystitis.

What is post-renal proteinuria?

200

Quality control is part of this phase of clinical laboratory testing.

What is analytical?

200

This type of monochromator can give you multiple different wavelengths in your spectrophotometer.

What is a diffraction grating?

200

Amino acids form zwitterions in solution. A term that describes this property with respect to acids and bases is this.

What is amphoteric?

200

This is very common condition that can result in both hyperalbuminemia and hyperproteinemia.

What is dehydration?

200

This is one of two dyes that can be used to measure urine protien concentration.

What is coomassie bue/pyrogallol red?

300

This is the lowest concentration of an analyte that can be reliably distinguished from zero.

What is the limit of detection?

300

This is the range of wavelengths transmitted by the monochromator with at least 50% of the transmittance of the nominal wavelength.

What is bandpass?

300

Peptide bonds form between these two functional groups.

What are amino groups and carboxyl groups?

300

This is a malignancy that can result in normal to low levels of albumin and very high levels of total protein.

What is multiple myeloma?

300

THis type of bacterial infection leads to high levels of protein in the CSF.

What is meningitis?

400

This process is known as establishing the mathematical relationship between a signal your are measuring and a concentration of an analyte.

What is calibration?

400

This type of spectrophotometric design is used to minimize fluctuations in the light source on absorbance readings. 

What is a double beam design?
400

These types of bonds stabilize alpha helices.

What are hydrogen bonds?

400

High levels of bilirubin or drugs in a patients serum can cause this type of erroneous reading for a BCG or BCP reagent system.

What is falsely decreased?

400

This condition leads to an increased protein level in CSF due to intrathecal synthesis of immunoglobulins.

What is multiple sclerosis?

500

Correlation of test results with other patient information is part of this phase of clinical laboratory testing.

What is post analytical?

500

This type of photodetector is the most sensitive to very low levels of light.

What is a photomultiplier tube?

500

This level of protein structure is not lost when a protein is denatured.

What is primary structure?

500

Prolonged tourniquet application before blood collection can lead to these two conditions. 

What is hyperproteinemia and hyperalbuminemia?

500

These are two disadvantages to using turbidimetric methods over dye binding methods for measuring CSF protein.

What are more limited photometric linearity and more interference from drugs and drug metabolites?

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