Nucleic Acid Isolation
The higher the elution volume, the _______ the yield, and the _______ the concentration.
The higher the elution volume, the higher the yield, and the lower the concentration.
These are the steps of PCR.
What is denaturation, annealing, and extension
These are the 2 main types of cancer related genes.
What are oncogenes and tumor suppressor genes
This is what you would call a repeating segment consisting of 4 base pairs.
What is a tetranucleotide repeat
This is the purpose of a ladder in gel electrophoresis.
What is to provide a standard to measure fragment size
Nucleic Acids bind to Cellulose Matrices under these conditions.
What is an enzyme that synthesizes DNA from an RNA template
What does SNP stand for?
Single Nucleotide Polymorphism
This is the definition of a tumor marker.
What is anything present in cancer cells that provides information about that cancer
In gel electrophoresis, how does gel concentration affect the separation of DNA fragments?
This is the correct order of steps for Nucleic Acid Isolation.
What is: Sample Disruption - Clearing Debris - Binding to Purification Matrix - Washing - Elution
What is chromosome 9 joining with chromosome 22
The C allele (Pro72) encodes this amino acid at codon 72.
What is Proline
Explain the difference between Germline mutations and Somatic mutations.
Germline mutations - inherited changes in DNA
Somatic mutations - changes in DNA that are acquired through lifetime
What is to monitor amplification in real time
Name two methods of physical sample disruption.
What are:
Sonication
Tissue Grinders
Bead-Beating
What is a "No RT Control" in an RT-PCR experiment
What is:
To locate genes associated with disease, drug response, and traits
In Lynch syndrome, this is the "second hit" required for cancer development.
What is loss of the remaining functional mismatch repair allele
There are approximately this many nucleotides in the human genome.
What is 3 billion
List three different purification formats.
What are:
Membranes, Slurries, Magnetic Particles, Columns, and Plates
While reviewing your BCR-ABL melt curve, you see two peaks. What causes two peaks?
What is non-specific amplification
This TP53 allele is more efficient at triggering apoptosis.
What is Arg72 (G allele)
If an STR locus has different allele lengths on two homologous chromosomes, that locus is called _______.
What is heterozygous
What is one base pair