Cell Structure
Humans have 46 chromosomes. How many do prokaryotic cells have?
One
The ability to cause disease represents...
Pathogenicity
kills all forms of microbial life
sterilization
Rolling a swab of a wound around on a slide is an example of this type of preparation.
Direct
Explain the use case of selective media
inhibits certain types of organisms
Gram positive organisms have the addition of this material that provides rigidity and stability.
Teichoic acid
These types of organisms wait for a decrease in host defense before infecting.
Opportunistic
Universal precautions can be defined as
treating every patient as if they are infectious
High dry refers to
40x
Hemolysis patterns are interpreted on this type of media.
Blood Agar
Prokaryotic DNA must be in this state to fit inside the cell
Supercoiled
The degree of damage caused to the host is...
Virulence
The single most useful practice to prevent transmission is
hand washing
Explain why immersion oil is used
Fill in the gap to increase resolution for 100x objective
I like increased amounts of CO2 so they call me a...
capnophile
This layer is much thicker in gram positive organisms
Peptidoglycan layer
This type of person has a pathogenic organism that has integrated into their normal flora.
Carriers
Describe negative pressure as it pertains to biological safety cabinets.
prevents infectious air from being recirculated prior to sterilization
List the steps of the gram stain procedure in order.
Fix the slide
Crystal violet
iodine
decolorize
safranin
I can survive in ambient air, but I much prefer there to be nitrogen to spare.
Aerotolerant anaerobes
This space is debatably only found in gram negative organisms.
Periplasmic space
These two types of pathogenic invasion allow for subsurface invasion of tissues
Enzymatic and forced phagocytic ingestion
Describe the highest level biosafety cabinet
BSL-3
fully enclosed
rubber gloves
negative pressure
What is iodine's responsibility in the gram stain process
act as a mordant
bond with crystal violet to create a more stable embedding into the gram positive wall
Colony morphology refers to...
Size, pigmentation, shape, density, texture, and consistency