Specimen Processing
Stains
Colony Morphology
Media
Bioterrorism
Organism Identification
100
Each of the following practices will better ensure the successful recovery of Campylobacter jejuni from stool specimens, except:
A. Incubation at 42C
B. Use of Campy BAP media
C. Use of selenite enrichment broth
D. Incubation in an atmosphere of 5% O2, 10% CO2, and 85% N2
C. Use of selenite enrichment broth
100
Report the gram stain.
Many GPC in pairs
Many WBC
100
Chocolate agar is recommended for the detection of Haemophilus species because it contains what?
Hemin and NAD
100
Which of these is not a responsibility of the sentinel micro labs?
A. rule out pathogens
B. determine that a bioterrorist event has occurred
C. transfer suspicious specimens to reference labs
D. be familiar with likely agents involved in a bio crime
B. determine that a bioterrorist event has occurred
100
The red arrow in pointing to a typical arrowhead-shaped zone of hemolysis that is produced by S. agalactiae with the CAMP test. A beta-hemolytic GPR that produces a rectangular-shaped area of hemolysis is:
Listeria monocytogenes
200
What is the STAT-iest specimen in the micro lab?
CSF
200
Report the gram stain.
Gram variable rods
200
This XLD plate shown in the following picture was isolated from a stool specimen of an immunocompetent adult. Your best action would be to:
Report it as no Salmonella or Shigella isolated
200
What media is CSF routinely plated to?
BAP, MAC, Chocolate, Thio
200
Describe a Category A bioterrorist agent
Cause high mortality and the potential for major public health impact
200
Strep pneumoniae can best be differentiated from the viridans group of Streptococci by which test?
Bile solubility
300
Which specimen type is inoculated by using a quantitative isolation method?
Urine
300
What is the interpretation of the following modified acid-fast stained specimen?
Acid-fast positive
300
The following picture is a gram stain from a positive anaerobic blood culture bottle. Some spores are seen on the gram stain. Based on the morphology, what is the most likely identification?
Clostridium septicum
300
Which of the following choices represents the characteristic indicator, fermentable sugar(s), and bacteriostatic agent(s) that are present in MacConkey agar respectively?
A. phenol red/lactose, sucrose, and xylose/sodium desoxycholate and bile salts
B. Eosin Y and methylene blue/lactose and sucrose/ Eosin Y and methylene blue
C. bromothymol blue/lactose, salicin, and sucrose/bile salts
D. neutral red/lactose/bile salts and crystal violet
D. neutral red/lactose/bile salts and crystal violet
300
A tech is working up a culture from an aspirated lesion. The culture is growing non-hemolytic colonies with flattened edges and raised centers, resembling a fried egg on BAP. Gram stain of the colonies show plump GNR with bipolar staining. What is the likely identification?
Yersinia pestis
300
An 87-year old patient had a foot wound that grew GNR. The colonies were pink to dark pink on the MacConkey agar, oxidase negative, and had the following biochemical reactions:
TSI: A/A
Indole: neg
MR: neg
VP: pos
Citrate: pos
H2S: neg
Urea: pos
Motility: neg
Ornithine: neg
AST: Ampicillin resistant, all others sensitive
A. Serratia marcescens
B. Proteus vulgaris
C. E. coli
D. Kleb pneumoniae
D. Kleb pneumoniae
400
A pleural fluid is received for aerobic culture. The specimen measures about 0.5 mL. How should this specimen be processed?
Use a sterile pipette and add a drop of fluid per agar plate.
400
Interpret the following image including the stain used in the preparation.
Fungal hyphae on KOH prep
400
Which of the following is a distinguising characteristic of Streptomyces species?
A. partial acid-fast organism
B. Resistance to lysozyme
C. musty basement odor
D. Salmon pink colonies
C. musty basement odor
400
What color would the agar be around a colony of Staph epi growing on MSA?
Red
400
What is one of the biggest challenges when attempting to isolate Francisella?
Organism growth is slow
400
All of the following statements regarding Enterobacteriaceae are true except:
A. They are all gram negative
B. They are all oxidase positive
C. They are all bacilli
D. They all ferment glucose
B. They are all oxidase positive
500
A swab is received for influenza testing on a cotton-tipped, wooden shaft swab, placed in a moist transport container. What is the appropriate next step?
Call the RN and let them know the specimen is not acceptable and needs to be recollected.
500
What is the presumptive identification of the following Wright Giemsa stained specimen?
Yeast cells consistent with Cryptococcus neoformans
500
Observation of colonies producing brick red fluorescence under long-wave UV light on KV allows for presumptive identification of which bacterial genus?
Prevotella
500
Which clostridium species is a potential bioterrorist agent?
Clostridium botulinum
500
Group A strep is best identified by what?
Bacitracin sensitivity