Omics overload
This branch of -omics focuses on all your DNA instructions.
What is genomics?
This lab technique is used to make millions of copies of a DNA sequence.
What is PCR?
This process introduces DNA, RNA, or other molecules into cells.
What is transfection?
This antibody-based test is used to detect proteins or antigens in samples.
What is ELISA?
This laser-based technique analyzes single cells in fluid.
What is flow cytometry?
This -omics field looks at every protein produced in a cell or tissue.
What is proteomics?
This machine rapidly changes temperatures to allow PCR reactions to occur.
What is a thermocycler?
This technique uses electric pulses to open temporary pores in cell membranes.
What is electroporation?
ELISAs are often run in these plastic plates with 96 small wells.
What are 96-well plates?
This specialized form of flow cytometry physically separates cells by type.
What is FACS?
This field studies the small molecules involved in metabolism.
What is metabolomics?
This PCR step allows short DNA sequences to bind to single strands of DNA.
What is annealing?
This lipid-based method is a chemical way to carry genes into cells.
What is lipofection?
This causes the color change that indicates a positive ELISA result.
What is a substrate reacting with an enzyme?
In HPLC, this is the liquid that carries the sample through the system.
What is the mobile phase?
This term describes the large-scale approach of studying all genes or proteins at once, not one at a time.
What is omics?
In rt-qPCR, this enzyme is used to convert RNA into DNA.
What is reverse transcriptase?
This type of hamster cell is used to mass-produce erythropoietin (EPO).
What are CHO cells?
In indirect ELISA, this antibody binds to the primary antibody and carries the enzyme.
What is the secondary antibody?
In HPLC, this solid part inside the column helps separate different molecules.
What is the stationary phase?
This personalized approach to treatment relies on omics data to tailor care to a person’s genes.
What is precision medicine?
In qPCR, this measurement reflects how much gene activity there was in the original sample.
What is fluorescence?
These engineered biological agents are used to deliver genes efficiently into difficult cells.
What are viral vectors?
This feature in the wells reveals how much antigen is present.
What is color intensity?
In this type of HPLC, smaller molecules get stuck in beads and take longer to come out.
What is size-exclusion HPLC?