CRIStory
The acronym CRISPR stands for this
What is "Clustered Regularly Interspaced Palindromic Repeats"?
This is what guides the Cas9 to the target site.
What is guide RNA?
This is the type of CRISPR that directly cuts out parts of DNA.
What is CRISPR Cas9-nickase?
The organism in which CRISPR DNA sequences were discovered
What is E. coli?
This is approximately how long a strand of sgRNA should be.
What is ~20 nucleotides long?
This is the type of CRISPR that involves edited epigenetics.
What is CRISPR dCas9-Tet1?
This is what the short palindromic repeats separate in bacterial DNA.
What are potentially dangerous viral genes?
Cas9 is this type of enzyme that cleaves DNA.
What is an endonuclease?
What is CRISPR Cas9-nickase?
CRISPR DNA in E. coli is hypothesized to serve this function due to the high content of foreign DNA in its repeats.
What is adaptive immunity in bacteria?
This molecule gives the CRISPR-Cas9 system its sequence specificity.
What is gRNA?
This is the type of CRISPR that does not alter the DNA, but instead works by demethylating cytosines to allow for transcription.
What is CRISPR dCas9-Tet1?
This RNA molecule allows bacteria to recognize and destroy viral DNA using sequence complementarity.
What is CRISPER RNA (crRNA)?
This enzyme demethylates cytosines at gene promoters, leading to activation of transcription.
What is Tet1?
What molecule and DNA repair process is used to introduce specific nucleotide edits into a genetic sequence?
What is a donor template and Homology-Directed Repair?