CRISPR Memory
What does CRISPR record?
Memory system
Also called "search and replace"?
Prime editing
What does gene therapy treat?
Diseases
How does bacteria remember a virus?
Viral DNA piece
What guides Cas9?
Guide RNA (gRNA)
Function of CRISPR memory?
Stores information
Main feature of prime editing?
Precise edits
How does gene therapy work?
Fixes genes
How is viral DNA stored?
Spacer in CRISPR
What is the role of gRNA in CRISPR?
Guides Cas9
What can CRISPR record to remember exposure to a toxin or virus?
Viral DNA piece
What does base editing do?
Changes single bases
What can cell therapy do?
Repairs tissue
How can CRISPR be used to model a viral infection?
Save viral DNA
How does Cas9 find where to cut?
gRNA + target
How do cells "remember" toxins?
DNA recording
What is a key benefit of base editing?
No double-strand breaks
What is an example of cell therapy?
Stem cell transplants
How do you model a genetic mutation with CRISPR?
gRNA + target
Where does Cas9 usually cut in the DNA?
3 bases before PAM
CRISPR as biological storage?
DNA storage
What is an advantage of prime editing over traditional CRISPR?
No double-strand breaks
What is the goal of gene therapy?
Correct genes
How is a mutation simulated in CRISPR modeling?
Guide RNA design
How does Cas9 locate its target site?
gRNA & PAM